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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Burkholderia cenocepacia UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Burkholderia cenocepacia UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)品名简称:
Recombinant Burkholderia cenocepacia lpxC protein货号:
CSB-EP451740BXS规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
lpxCUniprot No.:
B4E5Y5别名:
UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase种属:
Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))蛋白长度:
Full Length来源:
E.coli分子量:
51 kDa表达区域:
1-305aa氨基酸序列:
MLKQRTIKSIVKTVGIGVHSGRKIELTLRPAAPGTGIVFSRVDLPTPVDIPASAMSIGDTRLASVLQKDGVRVSTVEHLMSACAGLGIDNLYVDVTAEEIPIMDGSAATFVFLIQSAGIEEQNAPKRFIKVKKPVEIRDGDKFARLDPYFGFKLKFSIDFRHPAVDKTGQELEVDFATTSYVREIARARTFGFAHEAEMLREIGLARGGSMDNAIVLDEYRILNNDGLRYDDEFVKHKMLDAIGDLYVIGHPLLASYTAYKSGHGLNNALLRELLAHEDAYEIVTFDDPQAAPKGFAFDAQTAFA蛋白标签:
N-terminal 6xHis-SUMO-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Catalyzes the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis.功能2:
Catalyzes the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis.文献:
"The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patients." Holden M.T., Seth-Smith H.M., Crossman L.C., Sebaihia M., Bentley S.D., Cerdeno-Tarraga A.M., Thomson N.R., Bason N., Quail M.A., Sharp S., Cherevach I., Churcher C., Goodhead I., Hauser H., Holroyd N., Mungall K., Scott P., Walker D. Parkhill J. J. Bacteriol. 191:261-277(2009)研究领域:
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文献和实验A Method to Assay Inhibitors of Lipopolysaccharide Synthesis
include enzymes involved in the biosynthesis of lipopolysaccharides (LPS) that form outer membranes of these organisms. UDP-3-O -(R -3-hydroxymyristoyl)-N -acetylglucosamine deacetylase (LpxC) catalyzes the committed step in the biosynthesis of the lipid
领域奠基之作:Cell 重磅报道可胞外展示的糖基化修饰 RNA——glycoRNA
唾液酸形式 Neu5Ac 和 Neu5Gc。而当样品被 VC-Sia 或 RNase 处理时,则检测不到,进一步证实 glycoRNA 被唾液酸修饰的。 图片来源:Cell 经典的 N - 多糖合成机制有助于 glycoRNA 的产生 蛋白质上有两类主要的多糖,N - 多糖和 O - 多糖,两者都可以被唾液酸化。 研究人员使用遗传学、药理学和酶学的结合方法进行检测,探索 glycoRNA 结构是否与糖蛋白相关多糖结构相关。 IdID CHO 细胞系不能产生 UDP-Gal 和 UDP
kinases activate the serine/threonine kinases M n k l and M n k 2 . EMBO J. 16, 1909-1920. 13. Fujita, H., Fujita, H., Takemura, M., et al. (2003) A n ArabidopsisM A D S - b o x protein, A G L 2 4 , is specifically bound to and phosphorylated
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