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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Pseudomonas aeruginosa UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Pseudomonas aeruginosa UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)品名简称:
Recombinant Pseudomonas aeruginosa lpxC protein货号:
CSB-EP342468EZX规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
lpxCUniprot No.:
P47205别名:
UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase种属:
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)蛋白长度:
Full Length来源:
E.coli分子量:
49.4 kDa表达区域:
1-303aa氨基酸序列:
MIKQRTLKNIIRATGVGLHSGEKVYLTLKPAPVDTGIVFCRTDLDPVVEIPARAENVGETTMSTTLVKGDVKVDTVEHLLSAMAGLGIDNAYVELSASEVPIMDGSAGPFVFLIQSAGLQEQEAAKKFIRIKREVSVEEGDKRAVFVPFDGFKVSFEIDFDHPVFRGRTQQASVDFSSTSFVKEVSRARTFGFMRDIEYLRSQNLALGGSVENAIVVDENRVLNEDGLRYEDEFVKHKILDAIGDLYLLGNSLIGEFRGFKSGHALNNQLLRTLIADKDAWEVVTFEDARTAPISYMRPAAAV蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Catalyzes the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis.功能2:
Catalyzes the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis.文献:
"Cloning, expression, and purification of UDP-3-O-acyl-GlcNAc deacetylase from Pseudomonas aeruginosa: a metalloamidase of the lipid A biosynthesis pathway."Hyland S.A., Eveland S.S., Anderson M.S.J. Bacteriol. 179:2029-2037(1997)研究领域:
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文献和实验A Method to Assay Inhibitors of Lipopolysaccharide Synthesis
include enzymes involved in the biosynthesis of lipopolysaccharides (LPS) that form outer membranes of these organisms. UDP-3-O -(R -3-hydroxymyristoyl)-N -acetylglucosamine deacetylase (LpxC) catalyzes the committed step in the biosynthesis of the lipid
领域奠基之作:Cell 重磅报道可胞外展示的糖基化修饰 RNA——glycoRNA
唾液酸形式 Neu5Ac 和 Neu5Gc。而当样品被 VC-Sia 或 RNase 处理时,则检测不到,进一步证实 glycoRNA 被唾液酸修饰的。 图片来源:Cell 经典的 N - 多糖合成机制有助于 glycoRNA 的产生 蛋白质上有两类主要的多糖,N - 多糖和 O - 多糖,两者都可以被唾液酸化。 研究人员使用遗传学、药理学和酶学的结合方法进行检测,探索 glycoRNA 结构是否与糖蛋白相关多糖结构相关。 IdID CHO 细胞系不能产生 UDP-Gal 和 UDP
kinases activate the serine/threonine kinases M n k l and M n k 2 . EMBO J. 16, 1909-1920. 13. Fujita, H., Fujita, H., Takemura, M., et al. (2003) A n ArabidopsisM A D S - b o x protein, A G L 2 4 , is specifically bound to and phosphorylated
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