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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human cytomegalovirus Uncharacterized protein UL130 (UL130), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human cytomegalovirus Uncharacterized protein UL130 (UL130), partial品名简称:
Recombinant Human cytomegalovirus Uncharacterized protein UL130, partial货号:
CSB-EP325855HWV规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
UL130Uniprot No.:
P16772别名:
UL130Uncharacterized protein UL130种属:
Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)蛋白长度:
Partial来源:
E.coli分子量:
37.7 kDa表达区域:
26-214aa氨基酸序列:
SPWSTLTANQNPSPPWSKLTYSKPHDAATFYCPFLYPSPPRSPLQFSGFQQVSTGPECRNETLYLLYNREGQTLVERSSTWVKKVIWYLSGRNQTILQRMPQTASKPSDGNVQISVEDAKIFGAHMVPKQTKLLRFVVNDGTRYQMCVMKLESWAHVFRDYSVSFQVRLTFTEANNQTYTFCTHPNLIV蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
"The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome." Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J., McGeoch D.J., Hayward G.S. J. Gen. Virol. 84:17-28(2003)研究领域:
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文献和实验- 1.5g agarose in 130ml DDW. Boil to dissolve agarose and add 15ml 10 x MOPS. Cool and add 15ml formaldehyde. Add 3ul RNA and 7ul Sample buffer. Sample buffer (90ul total) = 50ul deionized formamide, 20ul formaldehyde, 10ul 10 x MOPS
The STINT‐NMR Method for Studying In‐cell Protein‐Protein Interactions
., and Pielak, G.J. 2008. Differential dynamical effects of macromolecular crowding on an intrinsically disordered protein and a globular protein: implications for in‐cell NMR spectroscopy. J. Am. Chem. Soc. 130:6310‐6311.
Affinity chromatography (with HIS-tagged proteins)
) Set up the column load sample. 50uL in vitro reaction (half the sample after the spin column), 130 uL AC buffer, and 45uL 5% milk powder. Total volume=225 uL 11)Wash the nickel resin. After the protein has been allowed to couple to the resin for 30
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