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UBE2D4 Rabbit pAb(bs-8354R)-50

ul/100ul/200ul
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  • ¥1180 - 2800
  • Bioss已认证
  • bs-8354R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    规格:200ul产品价格:¥2800.0
    产品编号bs-8354R
    英文名称UBE2D4 Rabbit pAb
    中文名称泛素蛋白连接酶D4抗体
    英文别名HBUCE 1; HBUCE 1; HBUCE1; UB2D4_HUMAN; UBE2D 4; UBE2D4; Ubiquitin carrier protein D4; Ubiquitin conjugating enzyme 1 isoform; Ubiquitin-conjugating enzyme E2 D4; Ubiquitin-protein ligase D4.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Rat (Mouse, Chicken, Pig, Cow, Horse, Sheep)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human UBE2D4
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量17 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    组织特异性Widely expressed.
    相似性Belongs to the ubiquitin-conjugating enzyme family.
    功能 Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity).
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (UBE1s), ubiquitin-conjugating enzymes (UBE2s), and ubiquitin-protein ligases (UBE3s). When ubiquitin is activated by a UBE1, it is transferred to the cysteine residue on a UBE2. UBE2 then binds a UBE3, which transfers the ubiquitin from the UBE2 cysteine to a lysine residue on the target protein. Ubiquitin-conjugating enzyme E2 Q1 (UBE2Q1), also known as ubiquitin-protein ligase Q1 or ubiquitin carrier protein Q1, is an 422 amino acid protein belonging to the ubiquitin-conjugating enzyme (UBE2) family. Two named isoforms of UBE2Q1 exist as a result of alternative splicing events.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded Rat ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with UBE2D4 Polyclonal Antibody, Unconjugated (bs-8354R) at 1:600 overnight at 4°C, DAB staining.
    产品细节图片2
    Sample: A549 Cell (Human) Lysate at 40 ug
    Primary: Anti-UBE2D4 (bs-8354R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 17 kD
    Observed band size: 18 kD

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