Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-RING finger protein 189 (bs-6872R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD
- ￥1180 - 2800
- Bioss
- bs-6872R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6872R |
| 英文名称 | RING finger protein 189 Rabbit pAb |
| 中文名称 | 环指蛋白189抗体 |
| 英文别名 | CARP-2; CARP2; Caspase regulator CARP2; Caspases 8 and 10 associated RING finger protein 2; Caspases-8 and-10-associated RING finger protein 2; E3 ubiquitin protein ligase rififylin; E3 ubiquitin-protein ligase rififylin; Fring; FYVE RING finger protein Sakura; FYVE-RING finger protein Sakura; Rffl; RFFL_HUMAN; Rififylin; RING finger and FYVE like domain containing protein; RING finger and FYVE-like domain-containing protein 1; RING finger protein 189; RING finger protein 34 like; RING finger protein 34-like; RNF189; RNF34L. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Pig, Cow, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human RING finger protein 189 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 40 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Binds CASP8 and CASP10. |
| 亚细胞定位 | Cytoplasm. Membrane; Peripheral membrane protein. |
| 组织特异性 | Ubiquitous. Detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. |
| 翻译后修饰 | Rapidly degraded after stimulation with TNFSF10, probably by caspases. Auto-ubiquitinated (in vitro). Palmitoylated. |
| 相似性 | Contains 1 FYVE-type zinc finger.
Contains 1 RING-type zinc finger. Contains 2 SAP domains. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Has E3 ubiquitin protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity. May bind phosphatidylinositol phosphates. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验相关实验
Studies of the Ubiquitin Proteasome System
and Translation Systems In Vitro Production of Ubiquitin‐Protein Ligases (E3s) Basic Protocol 24: Expression of Ring‐Finger E3s in Bacterial Lysates Alternate Protocol 14: Expression of HECT and U‐Box E3s in Bacterial Lysates Alternate Protocol 15: Expression
Measurement of Chloride Movement in Neuronal Preparations
Pearce, R.A. 1993. Physiological evidence for two distinct GABAA responses in rat hippocampus. Neuron 10:189‐200. Puia, G., Costa, E., and Vicini, S. 1994
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RING finger protein 189 Rabbit pAb(bs-6872R)-50ul/100ul/200ul
￥1180 - 2800
