Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti- CASC5 (bs-5959R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 265 kD
Observed band size: 265 kD
- ￥1180 - 2800
- Bioss
- bs-5959R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5959R |
| 英文名称 | CASC5 Rabbit pAb |
| 中文名称 | 肿瘤易感性候选基因5抗体 |
| 英文别名 | Protein D40/AF15q14; AF15Q14; ALL1 fused gene from chromosome 15q14; Blinkin bub linking kinetochore protein; Cancer susceptibility candidate 5; Cancer susceptibility candidate gene 5 protein; Cancer/testis antigen 29; CT29; D40; hKNL 1; hSpc105; KIAA1570; Kinetochore null 1 homolog; Kinetochore null protein 1; KNL1; CASC5_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CASC5 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 265 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Oncoproteins/suppressors > Oncoproteins Cell Biology > Cell Cycle > Cell Division > Centromere Stem Cells > Germline Stem Cells > Spermatogonial Stem Cells |
| 亚基 | Interacts with DSN1, MIS12, BUB1, BUB1B, NSL1 and ZWINT. |
| 亚细胞定位 | Nucleus. Chromosome, centromere, kinetochore. |
| 组织特异性 | Highly expressed in testis, where it is localized in germ cells, in particular in spermatocytes and in the pre-acrosome of round spermatids. Detected in the acrosome of ejaculated spermatozoa. Detected in adult thymus, bone marrow, colon, small intestine, appendix and placenta, and in fetal liver and thymus. |
| 功能 | Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Required for attachment of the kinetochores to the spindle microtubules. Directly links BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Four known isoforms exist. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CASC5) Polyclonal Antibody, Unconjugated (bs-5959R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验该产品被引用文献
[IF={{ 0 }}] {Lu, Zhimin, and Alfred WK Yung. "BIOMARKERS FOR CANCER CHARACTERIZATION AND TREATMENT." U.S. Patent No. 20,150,073,033. 12 Mar. 2015.} {IP} {=""}
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CASC5 Rabbit pAb(bs-5959R)-50ul/100ul/200ul
￥1180 - 2800
