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phospho-DDX5 (Tyr593) Rabbit p

Ab(bs-8005R)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-8005R
  • 2025年10月16日
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-8005R
    英文名称phospho-DDX5 (Tyr593) Rabbit pAb
    中文名称磷酸化三磷酸腺苷依赖解旋酶ddx5抗体
    英文别名DDX5(phospho Y593); DDX5(phospho Tyr593); p-DDX5(Y593); p-DDX5(Tyr593); ATP dependent RNA helicase DDX5; DDX 5; Ddx5; DDX5_HUMAN; DEAD(Asp Glu Ala Asp) box helicase 5; DEAD(Asp Glu Ala Asp) box polypeptide 5; DEAD box 5; DEAD box protein 5; DEAD/H(Asp Glu Ala Asp/His) box polypeptide 5(RNA helicase, 68kD); DEAD/H(Asp Glu Ala Asp/His) box polypeptide 5; DEAD/H(Asp-Glu-Ala-Asp/His) box polypeptide 5(RNA helicase 68kDa); DEAD/H box-5(RNA helicase 68kD); DKFZp686J01190; G17P1; HELR; HLR 1; HLR1; HUMP68; P68; P68; p68 RNA helicase; Probable ATP dependent RNA helicase DDX5; Probable ATP-dependent RNA helicase DDX5; RNA helicase 68kD; RNA helicase p68; RNA-dependent ATPase.
    产品应用WB=1:500-2000, IHC-P=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Rat (Mouse, Cow, Horse)
    抗体来源Rabbit
    免疫原KLH conjugated synthesised phosphopeptide derived from human DDX5 around the phosphorylation site of Tyr593
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量69 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Epigenetics and Nuclear Signaling > DNA / RNA > RNA Processing

    亚基Interacts with BRDT (By similarity). Identified in thespliceosome C complex. Interacts with RBM4; the interaction occursin a RNA-independent manner. Interacts with EIF2C1 and EIF2C2.Interacts with ESR1; the interaction is enhanced by phosphorylationof ESR1 AF-1 domain. Interacts with AR, NCOA1, NCOA2, NCOA3, EP300,CREBBP, POLR2A, TP53, RUNX2 and HDAC1. Self-associates. Interactswith DDX17. [INTERACTION] Q12873:CHD3; NbExp=4; IntAct=EBI-351962, EBI-523590;P45481:Crebbp (xeno); NbExp=3; IntAct=EBI-351962, EBI-296306;Q09472:EP300; NbExp=4; IntAct=EBI-351962, EBI-447295; P03372:ESR1;NbExp=8; IntAct=EBI-351962, EBI-78473; P22087:FBL; NbExp=6;IntAct=EBI-351962, EBI-358318; Q13547:HDAC1; NbExp=4;IntAct=EBI-351962, EBI-301834; O95983:MBD3; NbExp=4;IntAct=EBI-351962, EBI-1783068; P10085:Myod1 (xeno); NbExp=3;IntAct=EBI-351962, EBI-4405734; P24928:POLR2A; NbExp=3;IntAct=EBI-351962, EBI-295301; Q08775-3:Runx2 (xeno); NbExp=2;IntAct=EBI-351962, EBI-6119991; P04637:TP53; NbExp=3;IntAct=EBI-351962, EBI-366083; P04637-1:TP53; NbExp=2;IntAct=EBI-351962, EBI-3895849; P04637-7:TP53; NbExp=2;IntAct=EBI-351962, EBI-3895873.
    亚细胞定位Nucleus.
    翻译后修饰Arg-502 is dimethylated, probably to asymmetricdimethylarginine.
    Sumoylated; sumoylation, promoted by PIAS1, promotesinteraction with HDAC1 and transcriptional repression activity.Sumoylation also significantly increases stability, and reducespolyubiquitination.
    Polyubiquitinated, leading to proteasomal degradation.
    相似性Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    功能RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料p68 RNA helicase is a nuclear protein that exhibits RNA-dependent ATPase activity. Phosphorylation by protein kinase C inhibits p68 RNA helicase activity. p68 RNA helicase appears to play a role in organ differentiation during development. Furthermore, p68 RNA helicase is expressed in early neural development and in various mesodermal tissues in a number of different chordate embryos. At the cellular level, the expression levels of p68 RNA helicase increases in serum-induced quiescent cell lines. p68 RNA helicase may function as a coactivator for estrogen receptor alpha. Additionally, p68 RNA helicase associates with transcriptional coactivators CBP and p300. p68 RNA helicase localizes to the nucleus under normal conditions. During late telophase, p68 RNA helicase and fibrillarin colocalize to nascent nucleoli. p68 RNA helicase may function as a heterodimer with p72 RNA helicase.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-DDX5 (Tyr593)) Polyclonal Antibody, Unconjugated (bs-8005R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-DDX5 (Tyr593)) Polyclonal Antibody, Unconjugated (bs-8005R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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    [IF={{ 9.995 }}] {Alberto Gualtieri. et al. The RNA helicase DDX5 cooperates with EHMT2 to sustain alveolar rhabdomyosarcoma growth. CELL REP. 2022 Aug;40:111267} {WB} {Human}

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      。所以，我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体：Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本：石蜡包埋人类肺部肿瘤样本煮沸设备：浴锅 (左)，微波炉 (中)，高压锅 (右)免疫染色—封闭在 IHC–P 中，我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品，时长 1 小时，以防止非特异性背景染色。在进行 IHC–F 时，在含有 0.3% Triton. X–100 的 1X

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    phospho-DDX5 (Tyr593) Rabbit pAb(bs-8005R)-50ul/100ul/200ul
    ￥1180 - 2800