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- Bioss
- bs-11996R
- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-11996R |
| 英文名称 | Retinal S antigen Rabbit pAb |
| 中文名称 | 视网膜S抗原抗体 |
| 英文别名 | S-antigen; 48 kDa protein; Arrestin 1; Arrestin; ARRS_HUMAN; DKFZp686I1383; Retinal S antigen(48 KDa protein); Retinal S-antigen; Rod photoreceptor arrestin; RP47; S AG; S antigen; S antigen retina and pineal gland(arrestin); S antigen retina and pineal gland; S arrestin; S-AG; S-arrestin; SAG. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | (Human, Mouse, Rat, Dog, Cow, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Retinal S antigen |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 45 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Cytoplasmic and Plasma membrane |
| 组织特异性 | Retina and pineal gland. |
| 相似性 | Belongs to the arrestin family. |
| 功能 | Arrestin is one of the major proteins of the ros (retinal rod outer segments); it binds to photoactivated-phosphorylated rhodopsin, thereby apparently preventing the transducin-mediated activation of phosphodiesterase. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Members of the Arrestin/beta-Arrestin protein family are thought to participate in agonist-mediated desensitization of G protein-coupled receptors, and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters or sensory signals. Visual Arrestin, also known as Arrestin, retinal S-antigen or S-Arrestin, is a major soluble photoreceptor protein that regulates light-dependent signal transduction through G protein-coupled receptor (rhodopsin) activation. Visual Arrestin is expressed in retinal photoreceptor cells and the pineal gland. Visual Arrestin is the major pathogenic autoantigen in inflammatory eye disease, such as uveoretinitis and Oguchi disease, a rare autosomal recessive form of night blindness. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
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文献和实验Antigen Design Sera Purification Tech Sheet
will briefly explore peptide selection and design, coupling strategy, and carrier proteins which are important factors in anti-peptide antisera generation. Serum purification will also be discussed. For more complete coverage of antigen design, please refer
Antigen Design Sera Purification Tech Sheet
Sigma-Genosys routinely uses Freund's adjuvant for immunization purposes. The first injection is given in Complete Freund's adjuvant. Adjuvant is combined with the antigen to improve the immune response so that less vaccine is needed to produce
Polyclonal Antibody Production 简单快捷制备多抗方法，可用于western、ELISA和免疫/b
of a buffer such as phosphate buffered saline (PBS) or Tris that contains no chemicals harmful to the rabbit. 100 µg/rabbit of antigen is best, but you can use less and get good results. Again for two rabbits, prepare RIBI (or Freund's) adjuvant by warming
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