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50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-6961R |
| 英文名称 | ASMTY Rabbit pAb |
| 中文名称 | 松果体N甲基乙酰羟色胺抗体 |
| 英文别名 | acetylserotonin methyltransferase(Y chromosome); acetylserotonin N-methyltransferase; Acetylserotonin O-methyltransferase; ASMT; ASMTY; HIOM_HUMAN; HIOMT; HIOMTY; Hydroxyindole O-methyltransferase. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Dog, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Dopamine transporter |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 38 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Metabolism > Pathways and Processes > Metabolic signaling pathways > Amino acid metabolism Neuroscience > Neurotransmitter > Amino Acids Signal Transduction > Metabolism > Amino Acids |
| 组织特异性 | Expressed in the pineal gland (at protein level). In the retina, very low expression is found at the mRNA level (PubMed:7989373), and not at the protein level (PubMed:8574683). |
| 相似性 | Belongs to the methyltransferase superfamily. |
| 功能 | Produces melatonin (N-acetyl-5-methoxytryptamine) from N-acetylserotonin. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Most abundant in pineal gland, less in retina and brain, not in pigment epithelium. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |

Primary: Anti- ASMTY (bs-6961R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 38 kD
Observed band size: 38/45 kD
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文献和实验[IF={{ 5.249 }}] {Zhang, WL.. et al. Expression of arylalkylamine n-acetyltransferase (AANAT) and acetylserotonin o-methyltransferase (ASMT) in the corpus luteum of pregnant sows and synthesis of melatonin in luteal cells. 2021 Nov 24} {WB,IHC} {Pig}
[IF={{ 3.24 }}] {Herman, A. P., et al. "Central Interleukin-1β Suppresses the Nocturnal Secretion of Melatonin." Mediators of Inflammation 2016 (2016).} {WB} {="Others"}
[IF={{ 2.71 }}] {Herman, A. P., et al. "Interleukin-1β Modulates Melatonin Secretion in Ovine Pineal Gland: Ex Vivo Study." BioMed Research International (2015).} {WB} {="Other Species"}
[IF={{ 2.089 }}] {Huawei Wei. et al. Melatonin through blockade of Hif-1α signaling mediates the anti-fibrosis under hypoxia in canine Sertoli cells. REPROD BIOL. 2022 Dec;22:100677} {WB,IHC} {Dog}
[IF={{ 10.3 }}] {Jinho Kim. et al. Biosynthesis of neuroprotective melatonin is dysregulated in Huntington's disease. J PINEAL RES. 2023 Sep;:e12909} {WB} {Human,Mouse}
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
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