Blank control(blue): Hela Cells(fixed with 2% Paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-Phospholipase C beta 2/AF647 Conjugated antibody (bs-6976R-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
- ￥1180 - 2800
- Bioss
- bs-6976R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6976R |
| 英文名称 | Phospholipase C beta 1 Rabbit pAb |
| 中文名称 | 磷酯酶Cβ1抗体 |
| 英文别名 | 1-phosphatidylinositol-45-bisphosphate phosphodiesterase beta 1; 1vphosphatidylinositol 4, 5 bisphosphate phosphodiesterase beta 1; Phosphb; phosphoinositide phospholipase C; phosphoinositide phospholipase C-beta 1; phospholipase C beta 1(phosphoinositide-specific); phospholipase C I; PI PLC; PLC 1; PLCβ1; PLC-154; PLC-beta-1; PLC-I; PLC154; Plcb; PLCB1; Plcb1 protein; PLCbeta1. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Chicken, Dog, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Phospholipase C beta 1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 134 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Metabolism > Lipid metabolism Signal Transduction > Signaling Pathway > Lipid Signaling > PLC |
| 亚基 | Interacts with DGKQ. |
| 亚细胞定位 | Cytoplasmic and Nuclear. |
| 相似性 | Contains 1 C2 domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain. |
| 功能 | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Phospholipase C beta 1 catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. Its gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for its gene. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test} |
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospholipase C beta 1) Polyclonal Antibody, Unconjugated (bs-6976R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
U-2OS(Human) Cell Lysate at 30 ug
Primary: Anti- Phospholipase C beta 1 (bs-6976R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 134 kD
Observed band size: 134 kD
U-2OS(Human) Cell Lysate at 30 ug
Primary: Anti- Phospholipase C beta 1 (bs-6976R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 134 kD
Observed band size: 134 kD
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文献和实验该产品被引用文献
[IF={{ 5.7 }}] {Huifang Niu. et al. Molecular Mechanism of Pasteurized Akkermansia muciniphila in Alleviating Type 2 Diabetes Symptoms. J AGR FOOD CHEM. 2024;72(23):13083–13098} {WB} {Mouse}
[IF={{ 14.7 }}] {Li Yin. et al. Macrophage P2Y6R activation aggravates psoriatic inflammation through IL-27-mediated Th1 responses. ACTA PHARM SIN B. 2024 Jun;:} {WB} {Mouse}
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Phospholipase C beta 1 Rabbit pAb(bs-6976R)-50ul/100ul/200ul
￥1180 - 2800
