Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti-PPP2R3 (bs-5759R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
- ￥1180 - 2800
- Bioss
- bs-5759R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5759R |
| 英文名称 | PPP2R3 Rabbit pAb |
| 中文名称 | 蛋白质磷酸酶2A亚基3抗体 |
| 英文别名 | AI118493; DNA for thyroid hormone receptor binding site(258bp); P2R3A_HUMAN; PP2A subunit B B72/B130 isoforms; PP2A subunit B isoform PR72/PR130; PP2A subunit B isoform R3 isoform; PP2A subunit B isoforms B72/B130; PP2A subunit B PR72/PR130 isoforms; PP2A subunit B R3 isoform; Ppp2r3a; PR130; PR59; PR72; Serine/threonine protein phosphatase 2A 72/130 kDa regulatory subunit B; Serine/threonine-protein phosphatase 2A 72/130 kDa regulatory subunit B. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Dog, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PPP2R3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 130 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell cycle > Kinases/phosphatases > Phosphatases Cell Biology > Cell Cycle > Kinases/Phosphatases > Phosphatases Signal Transduction > Protein Phosphorylation > Ser / Thr Phosphatases |
| 亚基 | PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. |
| 组织特异性 | Expressed in heart, brain, placenta, lung, muscle and kidney. |
| 相似性 | Contains 2 EF-hand domains. |
| 功能 | The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP2R3) Polyclonal Antibody, Unconjugated (bs-5759R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
Skeletal muscle (Mouse) Lysate at 40 ug
Primary: Anti- PPP2R3 (bs-5759R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
Skeletal muscle (Mouse) Lysate at 40 ug
Primary: Anti- PPP2R3 (bs-5759R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti- PPP2R3 (bs-5759R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
Lung (Mouse) Lysate at 40 ug
Primary: Anti- PPP2R3 (bs-5759R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD
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PPP2R3 Rabbit pAb(bs-5759R)-50ul/100ul/200ul
￥1180 - 2800
