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PTP1B Recombinant Rabbit mAb(b

sm-60700R)-50ul/100ul/25ul
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  • ￥800 - 2500
  • Bioss已认证
  • bsm-60700R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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    • 规格：

      50ul/100ul/25ul

    规格：50ul产品价格：￥1400.0
    规格：100ul产品价格：￥2500.0
    规格：25ul产品价格：￥800.0
    产品编号bsm-60700R
    英文名称PTP1B Recombinant Rabbit mAb
    中文名称蛋白酪氨酸磷酸酶-1B重组兔单抗
    英文别名PTN1_HUMAN; Tyrosine-protein phosphatase non-receptor type 1; EC:3.1.3.48; PTPN1; Protein-tyrosine phosphatase 1B(PTP-1B);
    产品应用WB=1:500-2000, IHC-P=1:200-500, IHC-F=1:200-500, ICC/IF=1:50-200, IF=1:200-500, Flow-Cyt=1:50-100

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat
    抗体来源Rabbit
    免疫原A synthesized peptide derived from human PTPN1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Recombinant
    理论分子量50 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cardiovascular > Atherosclerosis > Diabetes associated

    Signal Transduction > Protein Phosphorylation > Tyrosine Phosphatases

    亚基Interacts with EPHA3 (phosphorylated); dephosphorylates EPHA3 and may regulate its trafficking and function.
    亚细胞定位Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=Interacts with EPHA3 at the cell membrane.
    组织特异性Most abundant in testis. Also found in kidney, spleen, muscle, liver, heart and brain.
    翻译后修饰Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.
    S-nitrosylation of Cys-215 inactivates the enzyme activity.
    Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.
    功能Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:200-500}
    {IHC-F}{1:200-500}
    {ICC/IF}{1:50-200}
    {IF}{1:200-500}
    {Flow-Cyt}{1:50-100}

     

    产品细节图片1
    25 ug total protein per lane of various lysates (see on figure) probed with PTP1B monoclonal antibody, unconjugated (bsm-60700R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated(bsm-60700R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated(bsm-60700R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated(bsm-60700R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated(bsm-60700R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
    产品细节图片6
    P‌‌araformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated(bsm-60700R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
    产品细节图片7
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated(bsm-60700R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
    产品细节图片8
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated(bsm-60700R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

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