Blank control: RSC96(blue), the cells were fixed with 2% Paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue),
Dilution: 1:200 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 0.2μg in 100 μL1X PBS containing 0.5% BSA(green).
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- 规格:
50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-11847R |
| 英文名称 | GPM6B Rabbit pAb |
| 中文名称 | 神经细胞膜糖蛋白M6b |
| 英文别名 | M6b; Neuronal membrane glycoprotein M6b; rhombex 29; GPM6B_HUMAN. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Pig, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GPM6B |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 29 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Developmental Biology > Organogenesis > Nervous system development Neuroscience > Neurology process > Neurogenesis |
| 亚基 | Interacts with SERT. |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein (By similarity). Note=Colocalizes with SERT at the plasma membrane |
| 组织特异性 | Neurons and glia; cerebellar Bergmann glia, in glia within white matter tracts of the cerebellum and cerebrum, and in embryonic dorsal root ganglia. |
| 相似性 | Belongs to the myelin proteolipid protein family. |
| 功能 | May be involved in neural development. Involved in regulation of osteoblast function and bone formation. Involved in matrix vesicle release by osteoblasts; this function seems to involve maintenance of the actin cytoskeleton. May be involved in cellular trafficking of SERT and thereby in regulation of serotonin uptake. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | GPM6B belongs to the myelin proteolipid protein family and is implicated in neural development. It is expressed in neurons and glia; cerebellar Bergmann glia, in glia within white matter tracts of the cerebellum and cerebrum, and in embryonic dorsal root ganglia. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1μg/Test} |


Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-GPM6B (bs-11847R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 36 kD
Primary: Anti-GPM6B (bs-11847R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 36 kD

Sample:
U251(Human) Cell Lysate at 30 ug
Primary: Anti-GPM6B (bs-11847R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 34 kD
U251(Human) Cell Lysate at 30 ug
Primary: Anti-GPM6B (bs-11847R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 34 kD
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GPM6B Rabbit pAb(bs-11847R)-50ul/100ul/200ul
¥1180 - 2800









