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50ul/100ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 产品编号 | bs-9878R |
| 英文名称 | Cyclophilin 40/PPID Rabbit pAb |
| 中文名称 | 亲环蛋白(亲环素)40抗体 |
| 英文别名 | 40 kDa peptidyl prolyl cis trans isomerase; 40 kDa peptidyl prolyl cis trans isomerase D; 40 kDa peptidyl-prolyl cis-trans isomerase; Cyclophilin D; Cyclophilin D; Cyclophilin related protein; Cyclophilin-40; Cyclophilin-related protein; Cyclophilin40; CyclophilinD; CYP 40; CYP 40; CYP-40; CYP40; CYP40; CYPD; CYPD; MGC33096; Peptidyl Prolyl Isomerase D; Peptidyl Prolyl Isomerase D; Peptidyl-prolyl cis-trans isomerase D; Peptidylprolyl isomerase D; PPIase; PPIase D; PPID; PPID; PPID_HUMAN; Rotamase; Rotamase D. |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Cyclophilin D |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 20, 40 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Binds ESR1. |
| 亚细胞定位 | Cytoplasm. |
| 组织特异性 | Widely expressed. |
| 相似性 | Belongs to the cyclophilin-type PPIase family. PPIase D subfamily. Contains 1 PPIase cyclophilin-type domain. Contains 3 TPR repeats. |
| 功能 | PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of cyclosporin A (CsA) and is encoded by a single unique gene conserved from yeast to humans. Cyclophilin B (CyPB) is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. Cyclophilin D (CyP40) is a widely expressed cytoplasmic protein that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. It is a widely expressed cytoplasmic protein. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ELISA} | {1:5000-10000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/test} |

Lane 1: Mouse NIH/3T3 cell lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Human HeLa cell lysates
Lane 5: Human 293T cell lysates
Lane 6: Human HepG2 cell lysates
Lane 7: Human K562 cell lysates
Lane 8: Human Jurkat cell lysates
Primary: Anti-Cyclophilin 40/PPID (bs-9878R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20, 40 kDa
Observed band size: 41 kDa
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文献和实验[IF={{ 1.7 }}] {Jinhua Wang. et al.Indirubin induces apoptosis in ovarian cancer cells via the mitochondrial pathway.AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH.2024 Oct 15;16(10):6119-6129.} {Western blot} {Human}
Generation of Spatio‐Temporally Controlled Targeted Somatic Mutations in the Mouse
Figure 1. Schematic structure of the K14‐Cre‐ERT2 transgene. The human K14 promoter, the Cre‐ERT2 coding sequence, and the simian virus 40 polyadenylation signal (polyA) are represented by blue, yellow, and purple boxes, respectively. The rabbit β
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