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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5577R |
| 英文名称 | phospho-PTPN6 (Tyr536) Rabbit pAb |
| 中文名称 | 磷酸化蛋白酪氨酸磷酸酶1C抗体 |
| 英文别名 | SHP-1(phospho-Tyr536); PTPN6(phospho Y536); SHP1(phospho Y536); SHP1; 70 kda SHP 1L protein; 70 kda SHP1L protein; 70Z-SHP; EC 3.1.3.48; HCP; HCPH; Hematopoietic cell phosphatase; Hematopoietic cell protein tyrosine phosphatase; Hematopoietic cell protein-tyrosine phosphatase; HPTP 1C; HPTP1C; MGC124580; Protein tyrosine phosphatase 1C; Protein tyrosine phosphatase non receptor type 6; Protein tyrosine phosphatase SHP 1; Protein tyrosine phosphatase SHP1; Protein-tyrosine phosphatase 1C; Protein-tyrosine phosphatase SHP-1; PTN6_HUMAN; PTP 1C; PTP-1C; PTP1C; PTPN 6; PTPN6; SH PTP 1; SH PTP1; SH-PTP1; SHP 1; SHP 1L; SHP1L; SHPTP 1; SHPTP1; Tyrosine protein phosphatase non receptor type 6; Tyrosine-protein phosphatase non-receptor type 6. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/test Not yet tested in other applications. |
| 交叉反应 | Human (Dog, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human PTPN6 around the phosphorylation site of Tyr536 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 65 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell cycle > Kinases/phosphatases > Phosphatases Cancer > Signal transduction > G protein signaling > GPCR Cell Biology > Cell Cycle > Kinases/Phosphatases > Phosphatases Developmental Biology > Organogenesis > Hematopoietic system development Signal Transduction > Signaling Pathway > G Protein Signaling > GPCR |
| 亚基 | Monomer. Interacts with MTUS1. Interacts with MILR1 (tyrosine-phosphorylated). Interacts with KIT. Binds PTPNS1, LILRB1 and LILRB2. Interacts with FCRL2, FCRL3, FCRL4, CD300LF, CDK2 and CD84. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts (via SH2 1 domain) with ROS1; the interaction is direct and promotes ROS1 dephosphorylation. Interacts with EGFR; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with LYN. Interacts with the tyrosine phosphorylated form of PDPK1. |
| 亚细胞定位 | Cytoplasm. Nucleus. Note=In neurons, translocates into the nucleus after treatment with angiotensin II. Shuttles between the cytoplasm and nucleus via its association with PDPK1. |
| 组织特异性 | Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells. |
| 翻译后修饰 | Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation. Phosphorylation at Tyr-564 enhances phosphatase activity. |
| 相似性 | Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains. Contains 1 tyrosine-protein phosphatase domain. |
| 功能 | Modulates signaling by tyrosine phosphorylated cellcsurface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {2ug/test} |
Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-PTPN6(Tyr564)) Polyclonal Antibody, Unconjugated (bs-5577R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
A549 Cell (Human) Lysate at 40 ug
MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti- phospho-PTPN6(Tyr564) (bs-5577R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
A549 Cell (Human) Lysate at 40 ug
MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti- phospho-PTPN6(Tyr564) (bs-5577R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
Sample: A431 Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-PTPN6(Tyr564)(bs-5577R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65kD
Observed band size: 65kD
Primary: Anti-phospho-PTPN6(Tyr564)(bs-5577R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65kD
Observed band size: 65kD
Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti- phospho-PTPN6(Tyr564) / AF647 Conjugated antibody (bs-5577R-AF647)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG- AF647 .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti- phospho-PTPN6(Tyr564) / AF647 Conjugated antibody (bs-5577R-AF647)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG- AF647 .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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phospho-PTPN6 (Tyr536) Rabbit pAb(bs-5577R)-50ul/100ul/200ul
￥1180 - 2800
