Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human A549 cell lysates
Primary: Anti-ATP6V0D2 (bs-12548R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
- ￥1180 - 2800
- Bioss
- bs-12548R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-12548R |
| 英文名称 | ATP6V0D2 Rabbit pAb |
| 中文名称 | ATP6V0D2蛋白抗体 |
| 英文别名 | V-ATPase D2; ATP6D2; ATPase H+transporting lysosomal 38kDa V0 subunit D; ATPase H+transporting lysosomal 38kDa V0 subunit D isoform 2; ATPase H+transporting lysosomal 38kDa V0 subunit D2; FLJ38708; V ATPase subunit d 2; Vacuolar ATP synthase subunit d 2; Vacuolar proton pump subunit d 2; VMA 6; VMA6; VA0D2_HUMAN. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog, Pig, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ATP6V0D2/V-ATPase D2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 40 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Metabolism > Plasma Membrane > ATPases |
| 亚基 | V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). |
| 亚细胞定位 | Apical plasma membrane. |
| 组织特异性 | Kidney, osteoclast and lung. |
| 相似性 | Belongs to the V-ATPase V0D/AC39 subunit family. |
| 功能 | Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A–H) make up the V1 domain and five subunits (a, d, c, c' and c") make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. V-ATPase D2 is a 350 amino acid protein that is expressed in kidney, lung and osteoclast. V-ATPase D2 has been implicated as a regulator of urine acidification, osteoclast fusion and bone formation. Furthermore, V-ATPase D2 has been identified as a dendritic cell marker. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验该产品被引用文献
[IF={{ 6.656 }}] {Yuxin Li. et al. Structure-based virtual screening for discovery of Paederosidic Acid from Paederia Scandens as novel P2Y14R antagonist. PHYTOMEDICINE. 2023 May;:154851} {WB} {Mouse}
[IF={{ 6.15 }}] {Fang, Chao. et al. Diterbutyl phthalate attenuates osteoarthritis in ACLT mice via suppressing ERK/c-fos/NFATc1 pathway, and subsequently inhibiting subchondral osteoclast fusion. Acta Pharmacol Sin. 2021 Aug;:1-12} {WB} {Mouse}
[IF={{ 5.051 }}] {Hasegawa Tomoka. et al. Evocalcet rescues secondary hyperparathyroidism-driven cortical porosity in chronic kidney disease male rats. ENDOCRINOLOGY. 2023 Jan;:} {IHC} {Rat}
[IF={{ 4.966 }}] {Hirohito Hirata. et al. PMEPA1 and NEDD4 control the proton production of osteoclasts by regulating vesicular trafficking. Faseb J. 2021 Feb;35(2):e21281} {IF} {Mouse}
[IF={{ 4.7 }}] {Ziyi Wang. et al. Macrophages Atp6v0d2 regulates XBP1-mediated cholesterol metabolism to suppress metabolic dysfunction-associated steatohepatitis progression. INT IMMUNOPHARMACOL. 2025 Aug;161:115088} {IF,WB} {Mouse}
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ATP6V0D2 Rabbit pAb(bs-12548R)-50ul/100ul/200ul
￥1180 - 2800
