Protein: k562(human) lysate;
Primary: rabbit Anti-Creatine Kinase MB (bs-14049R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 42 kD
Observed band size: 42 kD
- ￥1180 - 2800
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- bs-14049R
- 2025年10月16日
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-14049R |
| 英文名称 | Creatine Kinase MB Rabbit pAb |
| 中文名称 | 肌酸激酶同工酶CKMB抗体 |
| 英文别名 | CK-MB; B-CK; brain creatine kinase; CKBB; CKMB; CKMM; creatine kinase B; creatine kinase brain; creatine kinase M; Creatine kinase MB; Creatine Kinase MB type; creatine kinase muscle; M-CK; creatine kinase-M/B; KCRB_HUMAN; muscle creatine kinase; KCRM_HUMAN; |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Chicken, Dog, Pig, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Creatine Kinase MB |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 42 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Atherosclerosis > Diabetes associated Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism Metabolism > Types of disease > Diabetes Metabolism > Types of disease > Heart disease Signal Transduction > Metabolism > Energy Metabolism |
| 亚基 | Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. |
| 亚细胞定位 | Cytoplasm. |
| 相似性 | Belongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain. Contains 1 phosphagen kinase N-terminal domain. |
| 功能 | Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-Creatine Kinase MB (bs-14049R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-Creatine Kinase MB (bs-14049R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Uterus (Mouse) Lysate at 40 ug
Primary: Anti-Creatine Kinase MB (bs-14049R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Uterus (Mouse) Lysate at 40 ug
Primary: Anti-Creatine Kinase MB (bs-14049R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
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文献和实验该产品被引用文献
[IF={{ 5.195 }}] {Chuting Li. et al. Ginsenoside Rb1 promotes the activation of PPARα pathway via inhibiting FADD to ameliorate heart failure. EUR J PHARMACOL. 2023 May;947:175676} {WB} {Rat}
[IF={{ 1.3 }}] {Tao Zhong. et al. Electrochemical immunosensor based on gold nanoparticles for the detection of creatine kinase as a cardiac marker. INT J ELECTROCHEM SC. 2024 Nov;19:100821} {} {}
相关实验
infarction ,AMI）诊断最常用的三个指标：心肌肌钙蛋白（cardiac troponins，cTn）、肌酸激酶MB(creatine kinase MB, CK-MB)和肌红蛋白（myoglobin，Mb）。根据欧洲心脏病学会（European Society of Cardiology， ESC）/美国心脏病基金学院（American College of Cardiology Foundation，ACC）建议，心肌坏死诊断的生化标准为（1）在发病24小时内至少检测出一次cTnT或cTnI的最大
Developing Genetically Engineered Mouse Models to Study Tumor Suppression
., Trumbauer, M., Senear, A.W., Warren, R., and Palmiter, R.D. 1981. Somatic expression of herpes thymidine kinase in mice following injection of a fusion gene into eggs. Cell 27:223‐231.
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
grass group I allergenS, α-MF[124]Tomato prosysteminI, very low yield332Tomato Lycopesicon esculentum Mill. LeMir (L. esculentum miraculin)S, PHO1[125]Wheat lipid transfer proteinS, 720 mg/L, PHO1[126]Wheat puroindoline-a proteinS and Mb-bound, 14 mg/L
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Creatine Kinase MB Rabbit pAb(bs-14049R)-50ul/100ul/200ul
￥1180 - 2800
