Sample:
Lane 1: Recombinant human CH25H protein, His(bs-41322P)
Primary: Anti-CH25H (bs-6480R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: kDa
Observed band size: 11 kDa
- ￥1180 - 1980
- Bioss
- bs-6480R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 产品编号 | bs-6480R |
| 英文名称 | CH25H Rabbit pAb |
| 中文名称 | 胆固醇25羟化酶抗体 |
| 英文别名 | C25H; Cholesterol 25 hydroxylase; Cholesterol 25-hydroxylase; Cholesterol 25 monooxygenase; EC 1.14.99.38; EC=1.14.99.38; h25OH; CH25H_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CH25H |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 32 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of lipids and lipoproteins Cardiovascular > Lipids / Lipoproteins > Lipid Metabolism > Cholesterol Metabolism Cell Biology > Other Antibodies > Oxidative Stress Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Cholesterol Metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipid metabolism Metabolism > Pathways and Processes > Redox metabolism > Oxidative stress |
| 亚细胞定位 | Endoplasmic reticulum membrane |
| 翻译后修饰 | N-glycosylated. |
| 相似性 | Belongs to the sterol desaturase family. |
| 功能 | CH25H is involved in cholesterol and lipid metabolism and catalyses the formation of 25-hydroxycholesterol from cholesterol, leading to repression of cholesterol biosynthetic enzymes. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | CH25H (Cholesterol 25-hydroxylase), also known as h25OH, is a 272 amino acid endoplasmic membrane protein that belongs to the sterol desaturase family. CH25H contains clusters of histidine residues essential for catalytic activity and is involved in cholesterol and lipid metabolism. CH25H catalyzes the formation of 25-hydroxycholesterol from cholesterol leading to the repression of cholesterol biosynthetic enzymes. CH25H regulates lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. CH25H utilizes diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:400-800} |
| {IHC-F} | {1:400-800} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CH25H Polyclonal Antibody, Unconjugated (bs-6480R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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文献和实验该产品被引用文献
[IF={{ 7.971 }}] {Samantha A. Hutchinson. et al. Liver x receptor alpha drives chemoresistance in response to side-chain hydroxycholesterols in triple negative breast cancer. Oncogene. 2021 Mar;:1-12} {IHC} {Human}
[IF={{ 5.56 }}] {Mukherjee, Pallavi, et al. "Transgenic Tomatoes Expressing the 6F Peptide and Ezetimibe Prevent Diet-induced Increases of Interferon-β and Cholesterol 25-hydroxylase in Jejunum." Journal of Lipid Research (2017): jlr-M076554.} {IHC} {="Mouse"}
[IF={{ 4.122 }}] {Chattopadhyay et al. Treating the Intestine with Oral ApoA-I Mimetic Tg6F Reduces Tumor Burden in Mouse Models of Metastatic Lung Cancer. (2018) Sci.Rep. 8:9032} {IHC} {mouse}
[IF={{ 31.25 }}] {Ceglia, Simona. et al. An epithelial cell-derived metabolite tunes immunoglobulin A secretion by gut-resident plasma cells. NAT IMMUNOL. 2023 Jan;:1-14} {WB} {Mouse}
[IF={{ 12.8 }}] {Tran Thanh-Tam. et al. Disruption of cholesterol homeostasis triggers periodontal inflammation and alveolar bone loss. EXP MOL MED. 2023 Dec;:1-11} {IHC,WB} {Human,Mouse}
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CH25H Rabbit pAb(bs-6480R)-50ul/100ul
￥1180 - 1980
