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- bs-3061R
- 2026年10月13日
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- 技术资料
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3061R |
| 英文名称 | phospho-CEBP alpha (Thr222 + Thr226) Rabbit pAb |
| 中文名称 | 磷酸化转录调节因子CEBP α 抗体 |
| 英文别名 | CEBP alpha(phospho T226); p-CEBP alpha(phospho T226); CEBP alpha(phospho T222); p-CEBP alpha(phospho T222); Apoptotic cysteine protease; Apoptotic protease Mch 5; C/EBP alpha; CAP4; Caspase 8 precursor; CCAAT Enhancer Binding Protein alpha; CEBP; CEBP A; CEBP alpha; CEBPA; FADD homologous ICE/CED 3 like protease; FADD like ICE; FLICE; ICE like apoptotic protease 5; ICE8; MACH; MCH5; MORT1 associated CED 3 homolog; CEBPA_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken, Dog, Pig, Cow, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human CEBP alpha around the phosphorylation site of Thr222/226 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 38 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Signal transduction Cardiovascular > Lipids / Lipoproteins > Fatty Acids > Synthesis Epigenetics and Nuclear Signaling > Transcription > Other factors Epigenetics and Nuclear Signaling > Transcription > Transcription Factors Metabolism > Pathways and Processes > Redox metabolism > Fatty acid oxidation |
| 亚基 | Interacts with PRDM16. Binds DNA as a dimer and can form stable heterodimers with C/EBP beta and gamma. Interacts with UBN1. Interacts with HBV protein X. Interacts with ZNF638; this interaction increases transcriptional activation. |
| 亚细胞定位 | Nucleus |
| 相似性 | Belongs to the bZIP family. C/EBP subfamily._x000D_
Contains 1 bZIP domain. |
| 功能 | C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (rat pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEBPA) Polyclonal Antibody, Unconjugated (bs-3061R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEBPA) Polyclonal Antibody, Unconjugated (bs-3061R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEBPA) Polyclonal Antibody, Unconjugated (bs-3061R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
_x000D_ Lane 1: Human HepG2 cell lysates
_x000D_ Lane 2: Human MCF-7 cell lysates
_x000D_ Primary: Anti-Phospho-CEBP alpha (Thr222 + Thr226) (bs-3061R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 38 kDa
_x000D_ Observed band size: 44 kDa
_x000D_ _x000D_
_x000D_ Lane 1: Human HepG2 cell lysates
_x000D_ Lane 2: Human MCF-7 cell lysates
_x000D_ Primary: Anti-Phospho-CEBP alpha (Thr222 + Thr226) (bs-3061R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 38 kDa
_x000D_ Observed band size: 44 kDa
_x000D_ _x000D_
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CEBPA | CEBP-alpha (p-T222/T226) Rabbit pAb(bs-3061R)-50ul/100ul/200ul
￥1180 - 2800
