产品封面图

phospho-CEBP alpha (Thr222 + T

hr226) Rabbit pAb(bs-3061R)-50ul/100ul/200ul
收藏
  • ￥1180 - 2800
  • Bioss已认证
  • bs-3061R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
avatar
北京博奥森生物技术有限公司
品牌商
10 年钻石会员

  • 企业认证

    点击 QQ 联系

    • 相关产品推荐更多 >

    万千商家帮你免费找货

    0 人在求购买到急需产品
    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围：

      产品信息以Bioss网站为准

    • 规格：

      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-3061R
    英文名称phospho-CEBP alpha (Thr222 + Thr226) Rabbit pAb
    中文名称磷酸化转录调节因子CEBP α 抗体
    英文别名CEBP alpha(phospho T226); p-CEBP alpha(phospho T226); CEBP alpha(phospho T222); p-CEBP alpha(phospho T222); Apoptotic cysteine protease; Apoptotic protease Mch 5; C/EBP alpha; CAP4; Caspase 8 precursor; CCAAT Enhancer Binding Protein alpha; CEBP; CEBP A; CEBP alpha; CEBPA; FADD homologous ICE/CED 3 like protease; FADD like ICE; FLICE; ICE like apoptotic protease 5; ICE8; MACH; MCH5; MORT1 associated CED 3 homolog; CEBPA_HUMAN.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Chicken, Dog, Pig, Cow, Rabbit)
    抗体来源Rabbit
    免疫原KLH conjugated Synthesised phosphopeptide derived from human CEBP alpha around the phosphorylation site of Thr222/226
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量38 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Signal transduction

    Cardiovascular > Lipids / Lipoproteins > Fatty Acids > Synthesis

    Epigenetics and Nuclear Signaling > Transcription > Other factors

    Epigenetics and Nuclear Signaling > Transcription > Transcription Factors

    Metabolism > Pathways and Processes > Redox metabolism > Fatty acid oxidation

    亚基Interacts with PRDM16. Binds DNA as a dimer and can form stable heterodimers with C/EBP beta and gamma. Interacts with UBN1. Interacts with HBV protein X. Interacts with ZNF638; this interaction increases transcriptional activation.
    亚细胞定位Nucleus
    相似性Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.
    功能C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. [provided by RefSeq, Jul 2008].

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (rat pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEBPA) Polyclonal Antibody, Unconjugated (bs-3061R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEBPA) Polyclonal Antibody, Unconjugated (bs-3061R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEBPA) Polyclonal Antibody, Unconjugated (bs-3061R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片4
    Sample:
    Lane 1: Human HepG2 cell lysates
    Lane 2: Human MCF-7 cell lysates
    Primary: Anti-Phospho-CEBP alpha (Thr222 + Thr226) (bs-3061R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 38 kDa
    Observed band size: 44 kDa

    风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    相关实验

    • 【求助】磷酸化的Akt不见了！！

      (Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝

    • v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平

      4 ). 2. Other antibodies used were b-catenin (phospho-Thr41/Ser37/Ser33) (#9561; Cell Signaling Technology), b-catenin (#9562;Cell Signaling Technology), CRMP2 (pT514/509) (#PB-043; Kinasource, Dundee, Scotland) and CRMP2 (#AB-042;Kinasource). 3. Blocking buffer

    • v468 chapter 5 糖原合成酶激酶-3 的抑制

      antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San

    查看更多相关实验 >
    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    Phospho-CEBP alpha (Thr222 + Thr226)
    ￥780
    上海信裕生物科技有限公司
    2025年05月22日询价
    Phospho-CEBP alpha (Thr222 + Thr226)
    ￥780
    上海再康生物科技有限公司
    2025年11月20日询价
    Phospho-CEBP alpha (Thr222/226) Antibody
    ￥2400
    深圳市豪地华拓生物科技有限公司
    2025年07月09日询价
    Phospho-CEBPα(Thr222+Thr226)抗体
    ￥1380
    上海彩佑实业有限公司
    2025年06月02日询价
    Phospho-CEBP alpha (Thr222 + Thr226)磷酸化转录调节因子CEBP α 抗体
    ￥1380
    上海雅吉生物科技有限公司
    2025年07月08日询价
    phospho-CEBP alpha (Thr222 + Thr226) Rabbit pAb(bs-3061R)-50ul/100ul/200ul
    ￥1180 - 2800