SMTN | Smoothelin Rabbit pAb(bs-13740R)-50ul/100ul/200ul产品图
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SMTN | Smoothelin Rabbit pAb(b

s-13740R)-50ul/100ul/200ul
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  • ¥1180 - 2800
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  • bs-13740R
  • 2026年10月13日
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      50ul/100ul/200ul

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    规格:200ul产品价格:¥2800.0
    产品编号bs-13740R
    英文名称Smoothelin Rabbit pAb
    中文名称平滑肌蛋白抗体
    英文别名FLJ35168; FLJ35365; FLJ35620; FLJ38597; Smoothelin; SMSMO; Smtn; SMTN_HUMAN.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Rat (Mouse, Dog, Pig, Cow, Rabbit, Orangutan)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Smoothelin
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量100 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cardiovascular > Cardiovascular Markers > Cell Markers > Smooth Muscle Cells

    Cardiovascular > Vasculature > Smooth muscle cell (SMC) > Cytoskeleton

    Developmental Biology > Organogenesis > Skeletal development > Muscle

    Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Intermediate Filaments > Class III > Smoothelins

    亚细胞定位Cytoplasm > cytoskeleton. Exhibits a filamentous organization.
    组织特异性Smooth muscle; contractile or vascular (for the long form).
    相似性Belongs to the smoothelin family.
    _x000D_ Contains 1 CH (calponin-homology) domain.
    功能Structural protein of the cytoskeleton.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The cytoskeletal protein smoothelin is highly conserved among vertebrates and is expressed exclusively by contractile smooth muscle cells where it localizes to the filament network. Smoothelin associates with actin stress fibers but does not interact with desmin. At least two isoforms of smoothelin are produced by alternative splicing. The short isoform lacks amino acids 1–544 at the amino-terminus of the long isoform. The short isoform is expressed in visceral muscle tissue, including intestine and stomach, but not in brain, while the long isoform is expressed in all vascularized organs. In the vascular system, smoothelin expression is limited to large veins and arteries capable of pulsatile contraction. As a marker for the highly differentiated contractile smooth muscle cell, smoothelin expression is useful for studying vascular malformation and injury. The gene encoding human smoothelin maps to chromosome 22q12.2.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Smoothelin) Polyclonal Antibody, Unconjugated (bs-13740R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    产品细节图片2
    Sample:
    _x000D_ Lane 1: Human MCF-7 cell lysates
    _x000D_ Lane 2: Human K562 cell lysates
    _x000D_ Lane 3: Human Jurkat cell lysates
    _x000D_ Primary: Anti-Smoothelin (bs-13740R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 100 kDa
    _x000D_ Observed band size: 115 kDa

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    图标文献和实验
    该产品被引用文献

    [IF=5.4] Guoyong Zhang. et al. Buyang Huanwu Decoction suppresses cardiac inflammation and fibrosis in mice after myocardial infarction through inhibition of the TLR4 signalling pathway. J ETHNOPHARMACOL. 2023 Nov;:117388 IF Rat

    [IF=0.939] Prayitnaningsih et al. Neuropathy optic glaucomatosa induced by systemic hypertension through activation endothelin-1 signaling pathway in central retinal artery in rats. (2016) Int.J.Ophthalmol. 9:1568-1577 IF Rat

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    SMTN | Smoothelin Rabbit pAb(bs-13740R)-50ul/100ul/200ul
    ¥1180 - 2800