HOXA13 Rabbit pAb(bs-12244R)-50ul/100ul/200ul
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HOXA13 Rabbit pAb(bs-12244R)-5

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  • ￥1180 - 2800
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  • bs-12244R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-12244R
    英文名称HOXA13 Rabbit pAb
    中文名称同源异型盒基因HOXA13抗体
    英文别名Homeo box 1J; Homeo box A13; Homeobox 1J; Homeobox A13; Homeobox protein Hox A13; Homeobox protein Hox-1J; Homeobox protein Hox-A13; Homeobox protein HOXA13; Homeobox1J; HomeoboxA13; HOX 1; HOX 1J; Hox 1J protein; HOX A13; HOX1; HOX1J; HOXA 13; HOXA13; HXA13_HUMAN; Transcription factor HOXA13.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Chicken, Pig, Cow, Horse, Rabbit, Sheep)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from Human HOXA13
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量40 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Developmental Biology > Organogenesis > Excretory system development > Kidney development

    Epigenetics and Nuclear Signaling > Transcription > Domain Families > Developmental Families > HOX

    亚细胞定位Nucleus.
    相似性Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
    功能Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The Hox proteins play a role in development and cellular differentiation by regulating downstream target genes. Specifically, the Hox proteins direct DNA-protein and protein-protein interactions that assist in determining the morphologic features associated with the anterior-posterior body axis. HoxA13 and HoxD13 also bind to other BMP and TGF-beta/Activin-regulated Smad proteins including Smad1 and Smad2, but not Smad4. In humans and mice, loss of HOXA13 function causes defects in the growth and patterning of the digits and interdigital tissues. Analysis of HoxA13 expression reveals a pattern of localization overlapping with sites of reduced Bmp2 and Bmp7 expression in HoxA13 mutant limbs. HoxA13 regulates Bmp2 and Bmp7 expression, providing a link between HoxA13, its target genes and the specific developmental processes affected by loss of HoxA13 function.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    HOXA13 Rabbit pAb(bs-12244R)-5
    Tissue/cell: human placenta tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-HOXA13 Polyclonal Antibody, Unconjugated(bs-12244R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    HOXA13 Rabbit pAb(bs-12244R)-5
    Sample:
    Lane 1: Placenta (Mouse) Lysate at 40 ug
    Lane 2: Uterus (Mouse) Lysate at 40 ug
    Lane 3: Liver (Rat) Lysate at 40 ug
    Lane 4: Uterus (Rat) Lysate at 40 ug
    Lane 5: 293T (Human) Cell Lysate at 30 ug
    Lane 6: Hela (Human) Cell Lysate at 30 ug
    Lane 7: U2os (Human) Cell Lysate at 30 ug
    Primary: Anti-HOXA13 (bs-12244R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 40 kD
    Observed band size: 46 kD

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    [IF={{ 2.6 }}] {Bozkurt Ahmet Sarper. et al. The regenerative effect of exosomes isolated from mouse embryonic fibroblasts in mice created as a sciatic nerve crush injury model. MOL BIOL REP. 2024 Dec;51(1):1-14} {IHC} {Mouse}

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    HOXA13 Rabbit pAb(bs-12244R)-50ul/100ul/200ul
    ￥1180 - 2800