Blank control: Hep G2 Cells(blue),fixed with 2% Paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Primary Antibody: Rabbit Anti-MAP4K1/AF647 Conjugated antibody (bs-4134R/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
_x000D_
- ￥1180 - 2800
- Bioss
- bs-4134R
- 2026年10月13日
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- 技术资料
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-4134R |
| 英文名称 | MAP4K1 Rabbit pAb |
| 中文名称 | 造血祖细胞激酶1抗体 |
| 英文别名 | Hematopoietic progenitor kinase 1; Hematopoietic progenitor kinase; HPK1; Human Hematopoietic Progenitor Kinase; M4K1_HUMAN; Map4k1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; Mitogen Activated Protein Kinase Kinase Kinase Kinase 1; Mitogen-activated protein kinase kinase kinase kinase 1. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human MAP4K1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 91 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > MAPK Pathway Signal Transduction > Signaling Pathway > G Protein Signaling > Small G Proteins > Regulators |
| 亚基 | Interacts with MAP3K1. |
| 组织特异性 | Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. |
| 相似性 | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CNH domain. Contains 1 protein kinase domain. |
| 功能 | May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1μg/Test} |
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Sample:
_x000D_ U937(Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-MAP4K1 (bs-4134R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 91 kD
_x000D_ Observed band size: 91 kD
_x000D_
_x000D_ U937(Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-MAP4K1 (bs-4134R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 91 kD
_x000D_ Observed band size: 91 kD
_x000D_
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MAP4K1 | HPK1 Rabbit pAb(bs-4134R)-50ul/100ul/200ul
￥1180 - 2800
