Sample:
Lane 1: Thymus (Mouse) Lysate at 40 ug
Lane 2: Testis (Mouse) Lysate at 40 ug
Primary: Anti-CAMK4 (bs-6727R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 50 kD
- ￥1180 - 2800
- Bioss
- bs-6727R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6727R |
| 英文名称 | CAMK4 Rabbit pAb |
| 中文名称 | 钙/钙调蛋白依赖性蛋白激酶4抗体 |
| 英文别名 | Brain Ca(2+) calmodulin dependent protein kinase type 4; Brain Ca(2+) calmodulin dependent protein kinase type IV; Calcium/calmodulin dependent protein kinase type 4 catalytic chain; Calcium/calmodulin dependent protein kinase type IV catalytic chain; Calcium/calmodulin dependent protein kinase IV; Calcium/calmodulin dependent protein kinase type IV; CAM kinase 4; CAM kinase GR; CAM kinase IV; CaMK 4; CAMK GR; CaMK IV; CaMK4; CaMKGR; MGC36771. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog, Pig, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CAMKIV/CAMK4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 55 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Chromatin Modifying Enzymes > Phosphorylation Neuroscience > Neurotransmission > Calcium Signaling > Calmodulin / CaMK Signal Transduction > Signaling Pathway > Calcium Signaling > Calmodulin Pathway |
| 亚基 | Monomer (By similarity). Interacts with protein phosphatase 2A (PPP2CA/PPP2CB); the interaction is mutually exclusive with binding to Ca(2+)/calmodulin. |
| 亚细胞定位 | Cytoplasm. Nucleus. Note=Localized in hippocampal neuron nuclei. In spermatids, associated with chromatin and nuclear matrix (By similarity). |
| 组织特异性 | Expressed in brain, thymus, CD4 T-cells, testis and epithelial ovarian cancer tissue. |
| 翻译后修饰 | Phosphorylated by CaMKK1 and CaMKK2 on Thr-200. Dephosphorylated by protein phosphatase 2A. Autophosphorylated on Ser-12 and Ser-13. Glycosylation at Ser-189 modulates the phosphorylation of CaMK4 at Thr-200 and negatively regulates its activity toward CREB1 in basal conditions and during early inomycin stimulation. |
| 相似性 | Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain. |
| 功能 | Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consodilation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteolclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphrylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18. [CATALYTIC ACTIVITY] ATP + a protein = ADP + a phosphoprotein. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Ca2+/calmodulin dependent protein kinase type IV (CAMKIV) belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验该产品被引用文献
[IF={{ 4.4 }}] {Kai Tang. et al.Neoandrographolide inhibits mature osteoclast differentiation to alleviate bone loss and treat osteoporosis.FRONTIERS IN PHARMACOLOGY.2025 Feb 11:16:1466057.} {Western Blot} {Mouse}
[IF={{ 4.3 }}] {Qinbing Xue. et al. Lycorine (Lycoris radiata)—a unique natural medicine on breast cancer. J CELL MOL MED. 2024 Aug;28(16):e70032} {WB} {Human}
[IF={{ 4.12 }}] {Zuo et al. Alcohol amplifies ketamine-induced apoptosis in primary cultured cortical neurons and PC12 cells through down-regulating CREB-related signaling pathways. (2017) Sci.Rep. 7:10523} {WB} {Rat}
[IF={{ 3.173 }}] {Zuo D et al. Alcohol aggravates ketamine-induced behavioral, morphological and neurochemical alterationsin adolescent rats: The involvement of CREB-related pathways.Behav Brain Res. 2018 Sep 3;349:80-90.} {WB} {Rat}
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CAMK4 Rabbit pAb(bs-6727R)-50ul/100ul/200ul
￥1180 - 2800
