Tissue/cell: rat colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Slc22A17 Y Polyclonal Antibody, Unconjugated(bs-0444R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- bs-0444R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0444R |
| 英文名称 | Slc22A17 Rabbit pAb |
| 中文名称 | 可溶性载质转运蛋白22A17抗体 |
| 英文别名 | 24p3 receptor; 24p3R; SLC22A17; hBOIT; BOCT; BOIT; Brain type organic cation transporter; Brain-type organic cation transporter; Neutrophil gelatinase associated lipocalin receptor; Neutrophil gelatinase-associated lipocalin receptor; Lipocalin-2 receptor; NGAL receptor; NgalR; NGALR; NGALR2; NGALR3; OTTHUMP00000082690; OTTHUMP00000082691; S22AH_HUMAN; Slc22a17; Potent brain type organic ion transporter; Solute carrier family 22 member 17; Solute carrier family 22 organic cation transporter member 17. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Dog, Pig, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human NGALR |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 58 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Metabolism > Plasma Membrane > Channels |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein. Note=Upon LCN2-binding, it is internalized. |
| 组织特异性 | Expressed in brain. |
| 相似性 | Belongs to the major facilitator superfamily. Organic cation transporter family. |
| 功能 | Cell surface receptor for LCN2 (24p3) that plays a key role in iron homeostasis and transport. Able to bind iron-bound LCN2 (holo-24p3), followed by internalization of holo-24p3 and release of iron, thereby increasing intracellular iron concentration and leading to inhibition of apoptosis. Also binds iron-free LCN2 (apo-24p3), followed by internalization of apo-24p3 and its association with an intracellular siderophore, leading to iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration and resulting in apoptosis (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | SLC22A17 (solute carrier family 22 nember 17; organic cation transportor,).may act as a brain-specific organic ion transporter. The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
Lane 1: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 2: U2os (Human) Cell Lysate at 30 ug
Lane 3: U87MG (Human) Cell Lysate at 30 ug
Lane 4: A549 (Human) Cell Lysate at 30 ug
Lane 5: Siha (Human) Cell Lysate at 30 ug
Primary: Anti-Slc22A17 (bs-0444R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 61 kD
Lane 1: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 2: U2os (Human) Cell Lysate at 30 ug
Lane 3: U87MG (Human) Cell Lysate at 30 ug
Lane 4: A549 (Human) Cell Lysate at 30 ug
Lane 5: Siha (Human) Cell Lysate at 30 ug
Primary: Anti-Slc22A17 (bs-0444R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 61 kD
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文献和实验该产品被引用文献
[IF={{ 8 }}] {Xiangjie Fu. et al. Synergistic and Long-Lasting Wound Dressings Promote Multidrug-Resistant Staphylococcus Aureus-Infected Wound Healing. INT J NANOMED. 2023 Aug 16} {IHC} {Mouse}
[IF={{ 4.24 }}] {Wu, Limin, et al. "Lipocalin‐2 enhances angiogenesis in rat brain endothelial cells via reactive oxygen species and iron‐dependent mechanisms." Journal of Neurochemistry (2015).} {WB} {="Rat"}
[IF={{ 2.776 }}] {Du Y et al. Effects of lipocalin-2 on brain endothelial adhesion and permeability. PLoS One. 2019 Jul 3;14(7):e0218965.} {ICC} {Human}
[IF={{ 2.3 }}] {Rie Kido. et al. Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells. J ORAL BIOSCI. 2025 Mar;67:100624} {WB} {Human}
[IF={{ 10.199 }}] {Cui et al. NGALR is overexpressed and regulated by hypomethylation in esophageal squamous cell carcinoma. (2008) Clin.Cancer.Res. 14:7674-81} {IHC} {Human}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
, doi:10.1089/ten.TEA.2008.0444 (2009). 5. Achneck, H.E., et al. Regenerating titanium ventricular assist device surfaces after gold/palladium coating for scanning electron microscopy. Microsc. Res. Tech. 73, 71-76, doi:10.1002/jemt.20757
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Slc22A17 Rabbit pAb(bs-0444R)-50ul/100ul/200ul
￥1180 - 2800
