phospho-AKT1S1 (Thr246) Rabbit pAb(bs-3346R)-50ul/100ul/200ul

phospho-AKT1S1 (Thr246) Rabbit

pAb(bs-3346R)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-3346R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-3346R
    英文名称phospho-AKT1S1 (Thr246) Rabbit pAb
    中文名称磷酸化蛋白激酶AKT底物1抗体
    英文别名PRAS40(phospho Thr246); PRAS40(phospho T246); AKT1 substrate 1(proline rich); PRAS40; AKT1 substrate 1; AKT1-S1; AKT1S-1; AKT1S 1; AKT1 S1; Lobe; PRAS 40; PRAS-40; Proline rich akt substrate; 40 kDa proline rich AKT substrate; 40 kDa proline-rich AKT substrate; AKT1 substrate 1; AKTS1_HUMAN; MGC2865; Proline rich Akt substrate 40 kDa; Proline-rich AKT1 substrate 1.
    产品应用IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Rat (Human, Mouse, Dog, Cow, Horse)
    抗体来源Rabbit
    免疫原KLH conjugated Synthesised phosphopeptide derived from human PRAS40 around the phosphorylation site of Thr246
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量28 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cell Biology > Cell Cycle > Kinases/Phosphatases

    Neuroscience > Neurotransmission > Intracellular Signaling > Kinases

    Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases

    亚基Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Interacts directly with RPTOR. The phosphorylated form interacts with 14-3-3 proteins.
    亚细胞定位Cytoplasm, cytosol.
    组织特异性Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).
    翻译后修饰Phosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1.
    相似性Belongs to the ubiquitin-conjugating enzyme family.
    功能Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008]

     

    应用推荐稀释比例
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    phospho-AKT1S1 (Thr246) Rabbit
    P‌‌araformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Phospho-AKT1S1 (Thr246)) Polyclonal Antibody, Unconjugated (bs-3346R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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