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- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6656R |
| 英文名称 | Cyclin B2 Rabbit pAb |
| 中文名称 | 周期素B2抗体 |
| 英文别名 | Cyclin-B2; CycB2; G2 mitotic specific cyclin B2; G2/mitotic specific cyclin B2; CCNB2_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | (Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Cyclin B2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 45 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Cell Cycle > Cyclins > Cyclin B Family Epigenetics and Nuclear Signaling > Cell cycle > Cyclins > Cyclin B Family |
| 亚基 | Interacts with the CDK1 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. |
| 相似性 | Belongs to the cyclin family. Cyclin AB subfamily. |
| 功能 | Essential for the control of the cell cycle at the G2/M (mitosis) transition. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Cyclin B2 belongs to a the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin B2 has been shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
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文献和实验[IF={{ 5.6 }}] {Shuo Li. et al. Knockdown of DNMT1 Induces SLCO3A1 to Promote Follicular Growth by Enhancing the Proliferation of Granulosa Cells in Mammals. INT J MOL SCI. 2024 Jan;25(5):2468} {WB} {Human}
[IF={{ 2.65 }}] {Ruoyang Lin. et al. Inhibitory Effects of Rabdosia rubescens in Esophageal Squamous Cell Carcinoma: Network Pharmacology and Experimental Validation. EVID-BASED COMPL ALT. 2022 Nov 10;2022:2696347} {WB} {Human}
[IF={{ 2.08 }}] {Zhang, Jihong, et al. "Interleukin 18 augments growth ability via NF-κB and p38/ATF2 pathways by targeting cylin B1, cyclin B2, cylin A2, and Bcl-2 in BRL-3A rat liver cells." Gene (2015).} {WB} {="Rat"}
), GSK-3b (dilution1:1,000; 1 h at RT), or b-actin (dilution 1:10,000; 1 h at RT). 5. Wash the membrane three times for 5 min each in TBS-T. 6. Incubate the membrane for 1 h at RT with 30 mL of TBS-Tcontaining anti-mouse or anti-rabbit IgG
secondary antibody review -- data from 99 publications
whether any cell surface DFz2 had been internalized 4 flow cytometry localize of GPR30 in the endoplasmic reticulum 5 rhodamine immunocytochemistry detect kaposin B in SLK endothelial cells 6 IgG IgG2b Alexa Fluor 647 flow
Studies of the Ubiquitin Proteasome System
Assays of E1 and E2 Activity Basic Protocol 1: Thiolester Formation Between Rabbit E1 and Ubiquitin Basic Protocol 2: Thiolester Formation Between E2 and Ubiquitin Binding of Ubiquitin‐Proteasome Proteins Basic Protocol 3: Binding of E2s to E3s Alternate
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