Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
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Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
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Incubation: Anti-RNASE4/Angiogenin Polyclonal Antibody, Unconjugated(bs-6583R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
- ￥1180 - 2800
- Bioss
- bs-6583R
- 2026年10月13日
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- 技术资料
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6583R |
| 英文名称 | RNASE4 Rabbit pAb |
| 中文名称 | 核糖核酸酶4抗体 |
| 英文别名 | RAB1; Ribonuclease 4; Ribonuclease A B1; Ribonuclease, RNase A family, 4; RNAS4_HUMAN; RNase 4; RNS4. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Dog, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human RNASE4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 14 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Angiogenesis > Angiogenic Factors Epigenetics and Nuclear Signaling > DNA / RNA > RNA Processing |
| 亚细胞定位 | Secreted. |
| 相似性 | Belongs to the pancreatic ribonuclease family. |
| 功能 | This RNase has marked specificity towards the 3' side of uridine nucleotides. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene belongs to the pancreatic ribonuclease family. It plays an important role in mRNA cleavage and has marked specificity towards the 3' side of uridine nucleotides. Alternative splicing results in four transcript variants encoding the same protein. This gene and the gene that encodes angiogenin share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2013]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
Sample:
_x000D_ Lane 1: Human HepG2 cell lysates
_x000D_ Lane 2: Human HUVEC cell lysates
_x000D_ Primary: Anti-RNASE4 (bs-6583R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 14 kDa
_x000D_ Observed band size: 15 kDa
_x000D_ _x000D_
_x000D_ Lane 1: Human HepG2 cell lysates
_x000D_ Lane 2: Human HUVEC cell lysates
_x000D_ Primary: Anti-RNASE4 (bs-6583R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 14 kDa
_x000D_ Observed band size: 15 kDa
_x000D_ _x000D_
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RNASE4 | RNase 4 Rabbit pAb(bs-6583R)-50ul/100ul/200ul
￥1180 - 2800
