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- bs-3822R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3822R |
| 英文名称 | CCNG2 Rabbit pAb |
| 中文名称 | 周期素G2抗体 |
| 英文别名 | CCNG2_HUMAN; Cyclin-G2; Cyclin G2; CyclinG2; CCNG 2; CCNG-2; |
| 中文别名 | |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Dog, Pig, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CCNG2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 38 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Cell Cycle > Cyclins Cell Biology > Cell Cycle > Cyclins > Other Cyclins Epigenetics and Nuclear Signaling > Cell cycle > Cyclins > Other Cyclins |
| 亚细胞定位 | Cytoplasm. |
| 组织特异性 | High levels in cerebellum, thymus, spleen and prostate. Low levels in skeletal muscle. |
| 相似性 | Belongs to the cyclin family. Cyclin G subfamily. |
| 功能 | May play a role in growth regulation and in negative regulation of cell cycle progesssion. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Cyclin G2 may play a role in growth regulation and in negative regulation of cell cycle progesssion. Cyclin G2 expression levels increase through the cell cycle to peak in the mid/late-S phase and decrease during G2/M phase. It may also contribute in maintaining the quiescent state of differentiated cells. Cyclin G2 is similar to cyclin A in the cyclin box, although no kinase activity is detected. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {2ug/Test} |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclin G2) Polyclonal Antibody, Unconjugated (bs-3822R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample: Brain(Rat) lysate at 30ug;
Primary: Anti-Cyclin G2 (bs-3822R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 38kD
Observed band size : 38kD
May be formed of homo-dimer
Primary: Anti-Cyclin G2 (bs-3822R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 38kD
Observed band size : 38kD
May be formed of homo-dimer
Blank control:A431.
Primary Antibody (green line): Rabbit Anti-Cyclin G2 antibody (bs-3822R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-Cyclin G2 antibody (bs-3822R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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CCNG2 Rabbit pAb(bs-3822R)-50ul/100ul/200ul
￥1180 - 2800
