Sample:
Lane1: E-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug
Lane2: E-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug
Primary: Anti- E tag (bs-2116R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD
- ￥980 - 4000
- Bioss
- bs-2116R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 应用范围：
产品信息以Bioss网站为准
- 规格：
100ul/500ul
| 规格： | 100ul | 产品价格： | ￥980.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥4000.0 |
| 产品编号 | bs-2116R |
| 英文名称 | E Tag Rabbit pAb |
| 中文名称 | E-Tag标签抗体 |
| 英文别名 | E-Tag; |
| 产品应用 | WB=1:1000-5000 Not yet tested in other applications. |
| 交叉反应 | Species independent |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated E Tag peptide |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Tags & Cell Markers > Epitope Tags > Other Tags > E tag |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | E Tag is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized.Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-5000} |
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文献和实验相关实验
Western blot and gel overlays（蛋白印记与凝胶覆盖）
involving a secondary antibody. The secondary antibody is raised against the primary antibody, e.g. rabbit antibody is used to make an antibody in a goat - hence "goat anti-rabbit". This secondary antibody is chemically tagged, and the tag can involve
Studies of the Ubiquitin Proteasome System
Assays of E1 and E2 Activity Basic Protocol 1: Thiolester Formation Between Rabbit E1 and Ubiquitin Basic Protocol 2: Thiolester Formation Between E2 and Ubiquitin Binding of Ubiquitin‐Proteasome Proteins Basic Protocol 3: Binding of E2s to E3s Alternate
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
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E Tag Rabbit pAb(bs-2116R)-100ul/500ul
￥980 - 4000
