phospho-Mst1(Thr183) + Mst2(Thr180) Rabbit pAb(bs-3294R)-50ul/100ul/200ul
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phospho-Mst1(Thr183) + Mst2(Th

r180) Rabbit pAb(bs-3294R)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-3294R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-3294R
    英文名称phospho-Mst1(Thr183) + Mst2(Thr180) Rabbit pAb
    中文名称磷酸化蛋白激酶MST抗体
    英文别名MST1/MST2(phospho T183+T180); MST1/MST2(phospho T183); MST1/MST2(phospho Thr183); Kinase responsive to stress; Krs2; Mammalian STE20 like protein kinase 1; Mammalian STE20-like protein kinase 1; Mammalian sterile 20 like 1; MST-1; Serine/threonine kinase 4; Serine/threonine protein kinase Krs 2; Serine/threonine-protein kinase 4; Serine/threonine-protein kinase Krs-2; STE20 like kinase MST1; Serine/threonine-protein kinase 4 18kDa subunit; Serine/threonine-protein kinase 4 37kDa subunit; STE20-like kinase MST1; STK4; STK4_HUMAN; MST1/N; MST1/C; TIIAC; YSK3.
    产品应用WB=1:500-2000

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human (Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit)
    抗体来源Rabbit
    免疫原KLH conjugated Synthesised phosphopeptide derived from human Mst1 around the phosphorylation site of Thr183
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量19/37/56 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cardiovascular > Atherosclerosis > Apoptosis

    Cardiovascular > Heart > Hypertrophy

    Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases

    亚基Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation. Interacts with and stabilizes SAV1. Interacts with RASSF1. Interacts with FOXO3. Interacts with RASSF2 (via SARAH domain). Interacts with AR, PKB/AKT1, TNNI3 and SIRT1.
    亚细胞定位Cytoplasm. Nucleus. Note=The caspase-cleaved form cycles between the nucleus and cytoplasm.
    组织特异性Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Ubiquitously expressed.
    翻译后修饰Autophosphorylated on serine and threonine residues. Phosphorylation at Thr-120 and Thr-387 by PKB/AKT1, leads to inhibition of its: kinase activity, nuclear translocation and autophosphorylation at Thr-183. It also diminishes its cleavage by caspases and its ability to phosphorylate FOXO3.
    Proteolytically cleaved by caspase-3 during apoptosis at Asp-326 and Asp-349 resulting in a 37 kDa or a 39 kDa subunit respectively. The 39 kDa subunit is further cleaved into the 37 kDa form. Proteolytic cleavage results in kinase activation and nuclear translocation of the truncated form (MST1/N). It is less likely that cleavage at Asp-349 is a prerequisite for activation as this site is not conserved in the murine ortholog.
    相似性 Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SARAH domain.
    功能Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2. Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylates FOXO1 on 'Ser-212' and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner. Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1. Phosphorylates AR on 'Ser-650' and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq, Jul 2008]

     

    应用推荐稀释比例
    {WB}{1:500-2000}

     

    phospho-Mst1(Thr183) + Mst2(Th
    Sample:
    Hela(Human) Cell Lysate at 30 ug
    Primary: Anti-Phospho-Mst1(Thr183) + Mst2(Thr180) (bs-3294R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 19/37/56 kD
    Observed band size: 37 kD
    phospho-Mst1(Thr183) + Mst2(Th
    Sample:
    HepG2(Human) Cell Lysate at 30 ug
    Primary: Anti-Phospho-Mst1(Thr183) + Mst2(Thr180) (bs-3294R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 19/37/56 kD
    Observed band size: 37 kD
    phospho-Mst1(Thr183) + Mst2(Th
    Sample:
    293T(Human) Cell Lysate at 30 ug
    Primary: Anti-Phospho-Mst1(Thr183) + Mst2(Thr180) (bs-3294R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 19/37/56 kD
    Observed band size: 37 kD

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    图标文献和实验
    该产品被引用文献

    [IF={{ 5.589 }}] {Gao R et al. PM2.5-associated nitro-PAH exposure promotes tumor cell metastasis through Hippo-YAPmediated transcriptional regulation. Sci Total Environ. 2019 Aug 15;678:611-617.} {WB} {Human}

    [IF={{ 5 }}] {Seoyoung Choi. et al. Protective effect of melatonin against blue light-induced cell damage via the TRPV1–YAP pathway in cultured human epidermal keratinocytes. BIOFACTORS. 2025 Apr;51(2):e70015} {WB} {Human}

    [IF={{ 4.8 }}] {Pengfei Li. et al. IL-32 aggravates metabolic disturbance in human nucleus pulposus cells by activating FAT4-mediated Hippo/YAP signaling. INT IMMUNOPHARMACOL. 2024 Nov;141:112966} {WB,IHC} {Human}

    [IF={{ 4.645 }}] {Pin-Shi Ni. et al. Indirect regulation of HIPPO pathway by miRNA mediates high-intensity intermittent exercise to ameliorate aging skeletal muscle function. SCAND J MED SCI SPOR. 2023 Feb;:} {WB} {Rat}

    [IF={{ 4.5 }}] {Shouying Xu. et al. ARID1A restrains EMT and stemness of ovarian cancer cells through the Hippo pathway. INT J ONCOL. 2024 Aug;65(2):1-11} {WB} {Human}

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    文献支持
    phospho-Mst1(Thr183) + Mst2(Thr180) Rabbit pAb(bs-3294R)-50ul/100ul/200ul
    ￥1180 - 2800