Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ET-1 Polyclonal Antibody, Unconjugated(bsm-0954M) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(C-0010) staining
- ￥1180 - 5600
- Bioss
- bsm-0954M
- 2025年10月16日
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul/200ug
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 规格： | 200ug | 产品价格： | ￥5600.0 |
| 产品编号 | bsm-0954M |
| 英文名称 | ET-1(2C4) Mouse mAb |
| 中文名称 | 内皮素-1单克隆抗体 |
| 英文别名 | Big endothelin 1; EDN 1; EDN1; EDN1 protein; Endothelin1; ET 1; ET1; OTTHUMP00000039245; PPET1; Preproendothelin 1; Preproendothelin1 |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Dog, Pig, Cow, Horse, Sheep, GuineaPig) |
| 抗体来源 | Mouse |
| 免疫原 | KLH conjugated synthetic peptide derived from human ET-1(CSCSSLMDKECVYFCHLDIIW Disulfide bridges C1-C15, C3-C11) |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Monoclonal |
| 理论分子量 | 2.5/24 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Response to hypoxia Cardiovascular > Vasculature > Endothelium Cardiovascular > Vasculature > Vasoconstriction Metabolism > Pathways and Processes > Metabolism processes > Hypoxia Metabolism > Types of disease > Cancer Signal Transduction > Growth Factors/Hormones > Hormones |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. |
| 相似性 | Belongs to the endothelin/sarafotoxin family. |
| 功能 | Endothelins are endothelium-derived vasoconstrictor peptides. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. Endothelin 1 is expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.Molecular weight: 2491.9Molecular formula: C109H159N25O32S5CAS: 117399-94-7MDL: MFCD00133305Reconstitution: 1mM (1mg/0.4013mL) |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验该产品被引用文献
[IF={{ 3.54 }}] {Mai et al. Dyssynchronous pacing triggers endothelial-mesenchymal transition through heterogeneity of mechanical stretch in a canine model. (2014) Circ.. 79:201-9} {WB} {Canine}
[IF={{ 1.924 }}] {Di Cristofori A et al. Meningioma and Bone Hyperostosis: Expression of Bone Stimulating Factors and Review of the Literature.World Neurosurg. 2018 Jul;115:e774-e781.} {IHC} {Human}
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ET-1(2C4) Mouse mAb(bsm-0954M)-50ul/100ul/200ul/200ug
￥1180 - 5600
