Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti-CA II (bs-1709R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
- ￥1180 - 2800
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- bs-1709R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1709R |
| 英文名称 | CA II Rabbit pAb |
| 中文名称 | 碳酸酐酶2抗体 |
| 英文别名 | Carbonic anhydrase Ⅱ; CA 2; CA II; CA2; CAII; Car 2; Car2; Carbonate dehydratase II; Carbonic anhydrase 2; Carbonic anhydrase B; Carbonic anhydrase C; Carbonic anhydrase II; Carbonic dehydratase. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CA II |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 29 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Blood Cell Biology > Other Antibodies > Other Antibodies |
| 亚基 | Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. |
| 亚细胞定位 | Cytoplasm. |
| 相似性 | Belongs to the alpha-carbonic anhydrase family. |
| 功能 | Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20µM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis. Also known as: Carbonic anhydrase Ⅱ; CA 2; CA II; CA2; CAII; Car 2; Car2; Carbonate dehydratase II; Carbonic anhydrase 2 ; Carbonic anhydrase B; Carbonic anhydrase C; Carbonic anhydrase II; Carbonic dehydratase. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample:
Lane 1: Mouse Large intestine tissue lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Mouse Kidney tissue lysates
Lane 4: Mouse Cerebrum tissue lysates
Lane 5: Rat Large intestine tissue lysates
Lane 6: Rat Spleen tissue lysates
Lane 7: Rat Kidney tissue lysates
Lane 8: Rat Cerebrum tissue lysates
Primary: Anti-CA II (bs-1709R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 30 kDa
Lane 1: Mouse Large intestine tissue lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Mouse Kidney tissue lysates
Lane 4: Mouse Cerebrum tissue lysates
Lane 5: Rat Large intestine tissue lysates
Lane 6: Rat Spleen tissue lysates
Lane 7: Rat Kidney tissue lysates
Lane 8: Rat Cerebrum tissue lysates
Primary: Anti-CA II (bs-1709R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 30 kDa
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文献和实验该产品被引用文献
[IF={{ 3.297 }}] {Hongyi Qu. et al. The effects of vasoactive intestinal peptide on RANKL-induced osteoclast formation. Ann Transl Med. 2021 Jan; 9(2): 127} {WB} {Rat}
相关实验
V294.PART II，Chapter 2 Boyden法体外趋化试验
PART II：BASIC CELL MIGRATION AND RELATED ASSAYS vol.294 Guan J.-L. (ed.) Cell Migration-Developmental Methods and Protocols Chapter 2 Boyden Chamber Assay Summary The Boyden chamber assay, originally introduced by Boyden
protein depends upon the species that the antibody was raised in. Protein A binds well to rabbit, cat, human, pig and guinea pig IgG as well as mouse IgG2a and IgG2b. Protein G binds strongly to IgG from cow, goat, sheep, cow, horse, rabbit and guinea pig
Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
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CA II Rabbit pAb(bs-1709R)-50ul/100ul/200ul
￥1180 - 2800
