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50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-1709R |
| 英文名称 | CA II Rabbit pAb |
| 中文名称 | 碳酸酐酶2抗体 |
| 英文别名 | Carbonic anhydrase Ⅱ; CA 2; CA II; CA2; CAII; Car 2; Car2; Carbonate dehydratase II; Carbonic anhydrase 2; Carbonic anhydrase B; Carbonic anhydrase C; Carbonic anhydrase II; Carbonic dehydratase. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CA II |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 29 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Blood Cell Biology > Other Antibodies > Other Antibodies |
| 亚基 | Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. |
| 亚细胞定位 | Cytoplasm. |
| 相似性 | Belongs to the alpha-carbonic anhydrase family. |
| 功能 | Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20µM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis. Also known as: Carbonic anhydrase Ⅱ; CA 2; CA II; CA2; CAII; Car 2; Car2; Carbonate dehydratase II; Carbonic anhydrase 2 ; Carbonic anhydrase B; Carbonic anhydrase C; Carbonic anhydrase II; Carbonic dehydratase. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |

_x000D_ Bone (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-CA II (bs-1709R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 29 kD
_x000D_ Observed band size: 29 kD

_x000D_ Lane 1: Mouse Large intestine tissue lysates
_x000D_ Lane 2: Mouse Spleen tissue lysates
_x000D_ Lane 3: Mouse Kidney tissue lysates
_x000D_ Lane 4: Mouse Cerebrum tissue lysates
_x000D_ Lane 5: Rat Large intestine tissue lysates
_x000D_ Lane 6: Rat Spleen tissue lysates
_x000D_ Lane 7: Rat Kidney tissue lysates
_x000D_ Lane 8: Rat Cerebrum tissue lysates
_x000D_ Primary: Anti-CA II (bs-1709R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 29 kDa
_x000D_ Observed band size: 30 kDa
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文献和实验[IF=3.297] Hongyi Qu. et al. The effects of vasoactive intestinal peptide on RANKL-induced osteoclast formation. Ann Transl Med. 2021 Jan; 9(2): 127 WB Rat
Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1)
and a purified enzyme, best suited for studies of Ca2+ -ATPase activity are described. The two selected membranes are the human red blood cell (RBC) ghosts, a representative of plasma membranes (PM), and the rabbit skeletal muscle SR, an intracellular membrane
Muscle contraction is affected by the rapid release of Ca2+ ions through Ca2+ - conducting channels localized in an intracellular mem- brane compartment, the sarcoplasmic reticulum (SR). The rabbit skel- etal muscle SR Ca2+ release channel
Assessment of Intracellular Calcium in Cerebral Artery Myocytes
frequency and elevated global [Ca2+ ]i in cerebral artery myocytes using a rabbit model of subarachnoid hemorrhage. Here, we describe and discuss laboratory methods and procedures using the fluorescent Ca2+ indicator dyes fura-2 and fluo-4 to assess global
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