Blank control: Mouse spleen cells(fixed with 2% Paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody:Rabbit Anti-Piwil2 antibody(bs-3817R), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
- ￥1180 - 2800
- Bioss
- bs-3817R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3817R |
| 英文名称 | Piwil2 Rabbit pAb |
| 中文名称 | piwi样2蛋白抗体 |
| 英文别名 | PIWL2_HUMAN; Piwi-like protein 2; EC:3.1.26.-; HILI; CT80; mili; PIWIL1L; piwi like RNA-mediated gene silencing 2; Cancer/testis antigen 80(CT80); |
| 产品应用 | WB=1:500-2000, Flow-Cyt=0.2μg /Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog, Pig, Cow, Rabbit, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Piwil2/Mili |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 107 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > DNA / RNA > RNA Processing > RNAi Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > Nuclear Receptors > Nuclear Pore Complex Epigenetics and Nuclear Signaling > RNAi > Argonaute and Piwi Epigenetics and Nuclear Signaling > RNAi > RISC Stem Cells > Germline Stem Cells > Embryonic Germ Cells |
| 亚基 | Interacts with DDX4, MAEL, EIF3A, EIF4E, EIF4G, PRMT5 and WDR77. Associates with EIF4E- and EIF4G-containing m7G cap-binding complexes. Interacts (when methylated on arginine residues) with TDRD1. |
| 亚细胞定位 | Cytoplasm. Note=Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. |
| 组织特异性 | Expressed in adult testis and in most tumors. |
| 翻译后修饰 | Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule. |
| 相似性 | Belongs to the argonaute family. Piwi subfamily. Contains 1 PAZ domain. Contains 1 Piwi domain. |
| 功能 | Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | PIWIL2 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {0.2μg /Test} |
Sample:
Testis (Mouse) Lysate at 40 ug
Testis (Rat) Lysate at 40 ug
HepG2 (Human) Cell Lysate at 30 ug
Lovo (Human) Cell Lysate at 30 ug
293T (Human) Cell Lysate at 30 ug
A549 (Human) Cell Lysate at 30 ug
Primary: Anti-Piwil2 (bs-3817R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 107 kD
Observed band size: 110 kD
Testis (Mouse) Lysate at 40 ug
Testis (Rat) Lysate at 40 ug
HepG2 (Human) Cell Lysate at 30 ug
Lovo (Human) Cell Lysate at 30 ug
293T (Human) Cell Lysate at 30 ug
A549 (Human) Cell Lysate at 30 ug
Primary: Anti-Piwil2 (bs-3817R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 107 kD
Observed band size: 110 kD
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文献和实验该产品被引用文献
[IF={{ 3.64 }}] {Shi, Ran-ran, et al. "The immunogenicity of a novel cytotoxic T lymphocyte epitope from tumor antigen PL2L60 could be enhanced by 4-chlorophenylalanine substitution at position 1."?Cancer Immunology, Immunotherapy?(2013): 1-10.} {WB} {="Human"}
[IF={{ 3.49 }}] {Easley et al. Assessing reproductive toxicity of two environmental toxicants with a novel in vitro human spermatogenic model. (2015) Stem.Cell.Re. 14:347-55} {ICC} {Human}
[IF={{ 2.276 }}] {Tian Xiaohong. et al. 17-Allylamino-demethoxygeldanamycin Used Alone or in Combination with Sodium Orthovanadate Promotes Apoptosis and Inhibits Invasion of SH-SY5Y Cells by Modulating PIWIL2. Biomed Res Int. 2020;2020:7894712} {WB} {Human}
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Piwil2 Rabbit pAb(bs-3817R)-50ul/100ul/200ul
￥1180 - 2800
