Sample:
_x000D_
Cerebrum (Mouse) Lysate at 40 ug
_x000D_
Epithelium (Mouse) Lysate at 40 ug
_x000D_
Primary: Anti-PPP2R5E(bs-19971R)at 1/300 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 55kD
_x000D_
Observed band size: 55kD
_x000D_
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- 技术资料
- 规格:
50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-19971R |
| 英文名称 | PPP2R5E Rabbit pAb |
| 中文名称 | 蛋白磷酸酶2调节亚基5E抗体 |
| 英文别名 | 2A5E_HUMAN; Epsilon isoform of regulatory subunit B56 protein phosphatase 2A; PP2A B subunit B' epsilon; PP2A B subunit B' epsilon isoform; PP2A B subunit B56 epsilon; PP2A B subunit B56 epsilon isoform; PP2A B subunit isoform B''-epsilon; PP2A B subunit isoform B'-epsilon; PP2A B subunit isoform B56-epsilon; PP2A B subunit isoform PR61-epsilon; PP2A B subunit isoform R5-epsilon; PP2A B subunit PR61 epsilon; PP2A B subunit PR61 epsilon isoform; PP2A B subunit R5 epsilon; PP2A B subunit R5 epsilon isoform; PPP2R5E; Protein phosphatase 2 regulatory subunit B(B56) epsilon isoform; Protein phosphatase 2 regulatory subunit B' epsilon isoform; Regulatory subunit B of protein phosphatase 2 epsilon isoform; Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit epsilon. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PPP2R5E |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 55 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Neurotransmission > Intracellular Signaling > Phosphatases Signal Transduction > Protein Phosphorylation > Ser / Thr Phosphatases |
| 亚细胞定位 | Cytoplasm. |
| 翻译后修饰 | Phosphorylated on serine residues. |
| 相似性 | Belongs to the phosphatase 2A regulatory subunit B56 family. |
| 功能 | The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2013] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |


Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP2R5E) Polyclonal Antibody, Unconjugated (bs-19971R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Sample:
_x000D_ Testis (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti- PPP2R5E (bs-19971R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 55 kD
_x000D_ Observed band size: 55 kD
_x000D_
_x000D_ Testis (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti- PPP2R5E (bs-19971R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 55 kD
_x000D_ Observed band size: 55 kD
_x000D_

Sample:
_x000D_ Thymus (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti- PPP2R5E (bs-19971R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 55 kD
_x000D_ Observed band size: 55 kD
_x000D_
_x000D_ Thymus (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti- PPP2R5E (bs-19971R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 55 kD
_x000D_ Observed band size: 55 kD
_x000D_
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PPP2R5E | B56-epsilon Rabbit pAb(bs-19971R)-50ul/100ul/200ul
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