IGFBP4 | IBP-4 Rabbit pAb(bs-10585R)-50ul/100ul/200ul产品图
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IGFBP4 | IBP-4 Rabbit pAb(bs-1

0585R)-50ul/100ul/200ul
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  • bs-10585R
  • 2026年10月13日
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    北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-10585R
    英文名称IGFBP4 Rabbit pAb
    中文名称胰岛素样生长因子结合蛋白4抗体
    英文别名Insulin-like Growth Factor Binding Protein 4; BP 4; BP4; HT29 IGFBP; IBP 4; IBP4; IGF binding protein 4; IGFBP 4; IBP4_HUMAN.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Mouse, Rat (Human, Chicken, Pig, Cow, Horse, Sheep)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human IGFBP4
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量26 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cardiovascular > Angiogenesis > Growth Factors

    Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > Structures > Bone

    Signal Transduction > Growth Factors/Hormones > Insulin / Insulin-like

    Signal Transduction > Signaling Pathway > Lipid Signaling

    亚基Binds IGF2 more than IGF1.
    亚细胞定位Secreted.
    相似性Contains 1 IGFBP N-terminal domain.
    _x000D_ Contains 1 thyroglobulin type-1 domain.
    功能IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Insulin like growth factor binding protein 4 (IGFBP4) is produced from a DNA sequence encoding the human IGFBP4 protein. Mature human IGFBP4 has a calculated molecular mass of approximately 26 kDa. Due to glycosylation, the recombinant protein migrates as a 32 kDa and 25 kDa protein under reducing and non reducing conditions, respectively. Human IGFBP4 has a potential N-linked glycosylation site and shares approximately 90% amino acid sequence identity with both mouse and rat IGFBP4. IGFBP4 is a member of the superfamily of insulin-like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). IGFBP4 functions as an inhibitor of IGF action and its main function may be to protect cells from overstimulation by IGFs or to allow activation of alternate transmembrane signaling pathways that are inhibited by IGF exposure. IGFBP4 is expressed in multiple tissues including adrenal, testis, spleen, heart, lung, kidney, liver, stomach, hypothalamus, and brain cortex.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Tissue/cell: Rat spleen tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti-IGFBP4 Polyclonal Antibody, Unconjugated(bs-10585R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片2
    Tissue/cell: Rat adrenal tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti-IGFBP4 Polyclonal Antibody, Unconjugated(bs-10585R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片3
    Sample:
    _x000D_ Liver (Mouse) Lysate at 40 ug
    _x000D_ Primary: Anti-IGFBP4 (bs-10585R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 26 kD
    _x000D_ Observed band size: 27 kD

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    图标文献和实验
    该产品被引用文献
    [1]Low expression of IGFBP4 and TAGLN accelerate the poor overall survival of osteosarcoma.
    PMID:35665757Sci Rep2022-06-03
    图标技术资料

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    IGFBP4 | IBP-4 Rabbit pAb(bs-10585R)-50ul/100ul/200ul
    ￥1180 - 2800