Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_
Incubation: Anti-Telomerase Binding Protein, P23 Antibody, Unconjugated(bs-1909R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
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- 技术资料
- 规格:
50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-1909R |
| 英文名称 | Telomerase Binding Protein, P23 Rabbit pAb |
| 中文名称 | 端粒酶结合蛋白P23抗体 |
| 英文别名 | Telomerase Binding Protein, P23; Prostaglandin E synthase 3; Cytosolic prostaglandin E2 synthase; cPGES; Telomerase-binding protein p23; Hsp90 co-chaperone; Progesterone receptor complex p23; PTGES3; P23; TEBP; TEBP_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Telomerase Binding Protein, P23 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 18 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Hormone biosynthesis Epigenetics and Nuclear Signaling > Chromosome Structure > Telomeres Metabolism > Pathways and Processes > Endocrine metabolism > Hormone biosynthesis Metabolism > Types of disease > Cancer Signal Transduction > Metabolism > Lipid metabolism Signal Transduction > Protein Trafficking > Chaperones > Heat Shock Proteins |
| 亚基 | Binds to telomerase and to the progesterone receptor. |
| 亚细胞定位 | Cytoplasm. |
| 相似性 | Belongs to the p23/wos2 family._x000D_
Contains 1 CS domain._x000D_ |
| 功能 | Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes. Belongs to the p23/wos2 family. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |


Sample:
_x000D_ Jurkat(Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-Telomerase Binding Protein, P23 (bs-1909R) at 1/500 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 18 kD
_x000D_ Observed band size: 18 kD
_x000D_ Jurkat(Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-Telomerase Binding Protein, P23 (bs-1909R) at 1/500 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 18 kD
_x000D_ Observed band size: 18 kD
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PTGES3 | p23 Rabbit pAb(bs-1909R)-50ul/100ul/200ul
¥1180 - 2800






