Sample:
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Muscle (Mouse) Lysate at 40 ug
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Primary: Anti-MITF (bs-1990R) at 1/1000 dilution
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Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
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Predicted band size: 59 kD
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Observed band size: 57 kD
- ￥1180 - 2800
- Bioss
- bs-1990R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1990R |
| 英文名称 | MITF Rabbit pAb |
| 中文名称 | 微小细胞血症相关转录因子抗体 |
| 英文别名 | MITF_HUMAN; Microphthalmia-associated transcription factor; BHLHE32; Class E basic helix-loop-helix protein 32(bHLHe32); MI; WS2; CMM8; WS2A; COMMAD; MITF-A; |
| 中文别名 | |
| 产品应用 | WB=1:500-2000, ICC/IF=1:50-200 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Chicken, Dog, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human MITF |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 59 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper > HLH Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper > HLH / Leucine Zipper Immunology > Innate Immunity > Mast Cells |
| 亚基 | Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA in the form of homodimer or heterodimer with either TFE3, TFEB or TFEC. Interacts with KARS. |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. |
| 翻译后修饰 | Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter. Phosphorylated at Ser-180 and Ser-516 following KIT signaling, trigerring a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome._x000D_
Ubiquitinated following phosphorylation at Ser-180, leading to subsequent degradation by the proteasome. Deubiquitinated by USP13, preventing its degradation. |
| 相似性 | Belongs to the MiT/TFE family. Contains 1 basic helix-loop-helix (bHLH) domain. |
| 功能 | Transcription factor for tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) that plays a key role in melanocyte development. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ICC/IF} | {1:50-200} |
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (MITF) polyclonal Antibody, Unconjugated (bs-1990R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
although other techniques can be used. The choice of immobilized antibody binding protein depends upon the species that the antibody was raised in. Protein A binds well to rabbit, cat, human, pig and guinea pig IgG as well as mouse IgG2a and IgG2b . Protein G binds
Studies of the Ubiquitin Proteasome System
Assays of E1 and E2 Activity Basic Protocol 1: Thiolester Formation Between Rabbit E1 and Ubiquitin Basic Protocol 2: Thiolester Formation Between E2 and Ubiquitin Binding of Ubiquitin‐Proteasome Proteins Basic Protocol 3: Binding of E2s to E3s Alternate
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MITF Rabbit pAb(bs-1990R)-50ul/100ul/200ul
￥1180 - 2800
