Sample:
_x000D_
A431(Human) Cell Lysate at 30 ug
_x000D_
Primary: Anti-CK14+17+42+10 (bs-1792R) at 1/1000 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 52 kD
_x000D_
Observed band size: 52 kD
- ￥1180 - 2800
- Bioss
- bs-1792R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1792R |
| 英文名称 | CK14+17+42+10 Rabbit pAb |
| 中文名称 | 细胞角蛋白14抗体 |
| 英文别名 | Cytokeratin 14; CK 14; ck14; Cytokeratin14; Cytokeratin-14; Dowling Meara; ebs3; Epidermolysis bullosa simplex; k14; Keratin 14; Keratin type I cytoskeletal 14; Keratin14; Koebner; Krt 14; krt14; EBS3; EBS4; NFJ; Keratin, type I cytoskeletal 14; Cytokeratin 17; CK 17; ck17; Cytokeratin17; Cytokeratin-17; Keratin, type I cytoskeletal 17; Cytokeratin 10; CK 10; ck10; Cytokeratin10; Cytokeratin-10; Keratin, type I cytoskeletal 10; Cytokeratin 42; CK 42; ck42; Cytokeratin42; Cytokeratin-42; Keratin, type I cytoskeletal 42; K1C14_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CK14 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 52 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Intermediate Filaments > Class I > Cytokeratins |
| 亚基 | Heterotetramer of two type I and two type II keratins. keratin-14 associates with keratin-5. Interacts with TRADD and with keratin filaments. Associates with other type I keratins. |
| 亚细胞定位 | Cytoplasm. Nucleus. Note=Expressed in both as a filamentous pattern. |
| 组织特异性 | Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. |
| 翻译后修饰 | A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus. |
| 相似性 | Belongs to the intermediate filament family. |
| 功能 | The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
_x000D_ Esophageal (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-CK14+17+42+10 (bs-1792R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 52 kD
_x000D_ Observed band size: 52 kD
_x000D_ Esophageal (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-CK14+17+42+10 (bs-1792R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 52 kD
_x000D_ Observed band size: 52 kD
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文献和实验该产品被引用文献
[IF=5.391] Jiang Y et al. A mutation in MAP2 is associated with prenatal hair follicle density. ASEB J. 2019 Dec;33(12):14479-14490. IHC Pig
[IF=3.997] Hou R et al. Biological properties of a bionic scaffold for esophageal tissue engineering research. Colloids Surf B Biointerfaces. 2019 Apr 2;179:208-217. ICC,WB Rabbit
[IF=2.792] Yan, Wei. et al. Neural, adipocyte and hepatic differentiation potential of primary and secondary hair follicle stem cells isolated from Arbas Cashmere goats. BMC VET RES. 2022 Dec;18(1):1-18 IF,WB Goat
[IF=2.41] Yang Liu. et al. Down-regulation of Wnt10a affects odontogenesis and proliferation in mesenchymal cells. BIOCHEM BIOPH RES CO. 2013 May;434:717 Other ="Mouse"
[IF=0] Wang X et al. Differentiation of bMSCs on Biocompatible, Biodegradable, and Biomimetic Scaffolds for Largely Defected Tissue Repair. ACS Applied Bio Materials. 2019. WB,ICC Rabbit
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pan CK Rabbit pAb(bs-1792R)-50ul/100ul/200ul
￥1180 - 2800
