Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Human LO2 cell lysates
Primary: Anti-Lubricin (bs-11175R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 152 kDa
Observed band size: 160 kDa
- ￥1180 - 1980
- Bioss
- bs-11175R
- 2025年10月16日
- 产品信息以Bioss网站为准
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50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 产品编号 | bs-11175R |
| 英文名称 | Lubricin Rabbit pAb |
| 中文名称 | 巨核细胞刺激因子/蛋白多糖4/浅表层粘膜蛋白多糖抗体 |
| 英文别名 | Superficial zone proteoglycan; articular superficial zone protein; bG174L6.2; CACP; camptodactyly arthropathy coxa vara pericarditis syndrome gene; FLJ32635; HAPO; Jacobs camptodactyly-arthropathy-pericarditis syndrome gene; JCAP; megakaryocyte stimulating factor; MSF; PRG 4; PRG4; proteoglycan 4; Proteoglycan4; Superficial zone proteoglycan; SZP; PRG4_HUMAN. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Lubricin/SZP |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 152 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > Structures > Bone |
| 亚基 | Homodimer; disulfide-linked. |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Highly expressed in synovial tissue, cartilage and liver and weakly in heart and lung. Isoform B is expressed in kidney, lung, liver, heart and brain. Isoform C and isoform D are widely expressed. |
| 翻译后修饰 | N-glycosylated.
O-glycosylated; contains glycosaminoglycan chondroitin sulfate and keratan sulfate. The disulfide bond between Cys-1146 and Cys-1403 is essential for protein cleavage. |
| 相似性 | Contains 2 hemopexin-like domains. Contains 2 SMB (somatomedin-B) domains. |
| 功能 | Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.
Isoform F plays a role as a growth factor acting on the primitive cells of both hematopoietic and endothelial cell lineages. soluble molecule that acts as a carrier for insoluble surface-active phospholipid (SAPL). Depletion of lubricin function has been associated with camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), an arthritic-like autosomal recessive disorder. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Lubricin, also designated proteoglycan-4 or megakaryocyte stimulating factor, is important for boundary lubrication within articulating joints. It is a disulfide-linked homodimer (between Cysteine 1146 and Cysteine 1403) that is essential for protein cleavage. Lubricin inhibits synovial cell adhesion to the cartilage surface, but also prevents the deposition of proteins from synovial fluid onto cartilage. Lubricin is highly expressed in cartilage, liver and synovial tissue. Defects in the gene encoding for lubricin can cause Jakobs syndrome, also designated camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP). CACP is an autosomal recessive disorder characterized by joint failure associated with noninflammatory synoviocyte hyperplasia and subinitimal fibrosis of the synovial capsule. Lubricin undergoes different levels of glycosylation and may be detected at varying molecular weights. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验该产品被引用文献
[IF={{ 8.7 }}] {Yu Han. et al. High-precision bioactive scaffold with dECM and extracellular vesicles targeting 4E-BP inhibition for cartilage injury repair. MATER TODAY BIO. 2024 Aug;27:101114} {WB} {Rat}
[IF={{ 6.6 }}] {Lina Zhang. et al. YAP maintains cartilage stem/progenitor cell homeostasis in osteoarthritis. J ORTHOP TRANSL. 2024 May;46:79} {WB} {Rat}
[IF={{ 4.717 }}] {Liu C et al. Kartogenin enhances the therapeutic effect of bone marrow mesenchymal stem cells derived exosomes in cartilage repair. Nanomedicine (Lond). 2019 Dec 2.} {IHC} {Rat}
[IF={{ 19.924 }}] {Cairong Li. et al. Integrated and Bifunctional Bilayer 3D Printing Scaffold for Osteochondral Defect Repair. ADV FUNCT MATER. 2023 Feb;:2214158} {IF} {Rat}
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Lubricin Rabbit pAb(bs-11175R)-50ul/100ul
￥1180 - 1980
