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- bs-5971R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5971R |
| 英文名称 | Plzf Rabbit pAb |
| 中文名称 | 早幼粒细胞白血病锌指蛋白抗体 |
| 英文别名 | Promyelocytic leukemia zinc finger protein; ZBT16_HUMAN; ZBTB16; Zfp145; Zinc finger and BTB domain containing 16; Zinc finger and BTB domain-containing protein 16; Zinc finger protein 145(Kruppel like expressed in promyelocytic leukemia); Zinc finger protein 145; Zinc finger protein PLZF; Znf145. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Plzf |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 74 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Binds EPN1. Interacts with ZBTB32 and CUL3. |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung. |
| 相似性 | Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 1 BTB (POZ) domain. Contains 9 C2H2-type zinc fingers. |
| 功能 | Probable transcription factor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Probable transcription factor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
Primary Antibody (green line): Rabbit Anti-PLZF antibody (bs-5971R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验[IF={{ 6.975 }}] {Meng-Fei Zhang. et al. Transcription factor Dmrt1 triggers the SPRY1-NF-κB pathway to maintain testicular immune homeostasis and male fertility. ZOOL RES. 2023 May 18; 44(3): 505–521} {IF} {Mouse}
[IF={{ 6.208 }}] {Baohui Yao. et al. Reproductive Suppression Caused by Spermatogenic Arrest: Transcriptomic Evidence from a Non-Social Animal. INT J MOL SCI. 2023 Jan;24(5):4611} {IHC} {Zokor}
[IF={{ 5.076 }}] {Feng T et al. Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis PathwayOxid Med Cell Longev.2020 Dec 31;2020:5954635.} {IF} {Goat}
[IF={{ 4.8 }}] {Meng-fei ZHANG. et al. MiR-21-5p ameliorates Busulfan-induced testicular dysfunction and maintains spermatogenesis1. J INTEGR AGR. 2024 Feb;:} {IF} {Goat}
[IF={{ 3.26 }}] {Niu, Zhiwei, et al. "Ras/ERK1/2 pathway regulates the self-renewal of dairy goat spermatogonia stem cells." Reproduction 149.5 (2015): 445-452.} {Other} {="Goat"}
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