Sample: Placenta (Mouse) Lysate at 40 ug
_x000D_
Primary: Anti-GCNT2(bs-10196R) at 1/300 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 46 kD
_x000D_
Observed band size: 56 kD
_x000D_
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- 技术资料
- 规格:
50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-13316R |
| 英文名称 | GCNT2 Rabbit pAb |
| 中文名称 | β1-6 N-乙酰氨基葡萄糖转移酶2抗体 |
| 英文别名 | bA360O19.2; bA421M1.1; beta 1 6 N acetylglucosaminyltransferase 2; CCAT; GCNT 2; GCNT2C; GCNT5; Glucosaminyl(N acetyl) transferase 2 I branching enzyme(I blood group); Glucosaminyl(N acetyl) transferase 2 I branching enzyme; I beta 1 6 N acetylglucosaminyltransferase; I branching beta 1 6 acetylglucosaminyltransferase; I branching enzyme; IGNT; II; Ii blood group; MGC163396; N acetylglucosaminyltransferase; N acetyllactosaminide beta 1 6 N acetylglucosaminyltransferase; NACGT1; NAGCT1; ULG3. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GCNT2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 46 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > ECM Proteins > Glucosaminoglycans Stem Cells > Hematopoietic Progenitors > Myeloid > Erythrocytic Lineage |
| 亚细胞定位 | Golgi apparatus membrane. |
| 组织特异性 | In the adult, highly expressed in prostate and to a lesser extent in small intestine and colon. Barely detected in heart, brain, kidney and pancreas. No expression in placenta, lung, liver, skeletal muscle, spleen, thymus, testis, ovary and peripheral blood leukocytes. In fetus, highly expressed in brain and to a lesser extent in lung and kidney. Barely detected in liver. |
| 相似性 | Belongs to the glycosyltransferase 14 family. |
| 功能 | GCNT2 is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase that converts linear into branched poly-N-acetyllactosaminoglycans and is responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding 3 different isoforms have been described. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Belonging to the glycosyltransferase 14 family, GCNT2 (glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group)), also known as II, N-acetylglucosaminyltransferase, IGNT, CCAT, ULG3, GCNT5, GCNT2C or NACGT1, is a 400 amino acid glycosyltransferase that localizes to the Golgi apparatus. Other members of the glycosyltransferase 14 family include GCNT1, GCNT3, GCNT4, GCNT6 and GCNT7. A single-pass type II membrane protein, GCNT2 functions as a branching enzyme known as beta-1,6-N-acetylglucosaminyltransferase, which converts fetal i antigen to adult I antigen in erythrocytes during embryonic development. With expression levels increasing significantly during oncogenesis and development, GCNT2 is found at highest levels in adult prostate and fetal brain, and is found at low levels in heart, small intestine, colon, brain, pancreas and kidney. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |


Sample:
_x000D_ Lane 1: Rat Cerebrum tissue lysates
_x000D_ Lane 2: Human A431 cell lysates
_x000D_ Primary: Anti-GCNT2 (bs-13316R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 46 kDa
_x000D_ Observed band size: 46 kDa
_x000D_ _x000D_
_x000D_ Lane 1: Rat Cerebrum tissue lysates
_x000D_ Lane 2: Human A431 cell lysates
_x000D_ Primary: Anti-GCNT2 (bs-13316R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 46 kDa
_x000D_ Observed band size: 46 kDa
_x000D_ _x000D_
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GCNT2 | IGNT Rabbit pAb(bs-13316R)-50ul/100ul/200ul
¥1180 - 2800









