Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_
Incubation: Anti-PAX2 Polyclonal Antibody, Unconjugated(bs-1187R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
- ￥1180 - 2800
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- bs-1187R
- 2026年10月13日
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1187R |
| 英文名称 | PAX2 Rabbit pAb |
| 中文名称 | 配对盒基因2抗体 |
| 英文别名 | PAX2 Paired box gene 2; Paired box gene 2; paired box homeotic gene 2; paired box protein 2; Paired box protein Pax 2; Paired box protein Pax-2; Paired box protein Pax2; Pax 2. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PAX2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 46 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Developmental Biology > Organogenesis > Excretory system development > Kidney development Epigenetics and Nuclear Signaling > Transcription > Domain Families > Developmental Families > PAX Epigenetics and Nuclear Signaling > Transcription > Transcription Factors Neuroscience > Cell Type Marker > Neural Stem Cell marker |
| 亚基 | Interacts with ELGN3; the interaction targets PAX2 for destruction. |
| 亚细胞定位 | Nuclear. |
| 组织特异性 | Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system. |
| 相似性 | Contains 1 paired domain. |
| 功能 | Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor supressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
Blank control（black line）:HepG2.
_x000D_ Primary Antibody (green line): Rabbit Anti-PAX2 antibody (bs-1187R)
_x000D_ Dilution:1ug/Test;
_x000D_ Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
_x000D_ Dilution: 0.5ug/Test.
_x000D_ Isotype control（orange line）: Normal Rabbit IgG
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
_x000D_ Primary Antibody (green line): Rabbit Anti-PAX2 antibody (bs-1187R)
_x000D_ Dilution:1ug/Test;
_x000D_ Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
_x000D_ Dilution: 0.5ug/Test.
_x000D_ Isotype control（orange line）: Normal Rabbit IgG
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Sample:
_x000D_ Lane 1: Mouse Kidney tissue lysates
_x000D_ Lane 2: Mouse Cerebellum tissue lysates
_x000D_ Lane 3: Rat Cerebellum tissue lysates
_x000D_ Primary: Anti-PAX2 (bs-1187R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 46 kDa
_x000D_ Observed band size: 47 kDa
_x000D_ Lane 1: Mouse Kidney tissue lysates
_x000D_ Lane 2: Mouse Cerebellum tissue lysates
_x000D_ Lane 3: Rat Cerebellum tissue lysates
_x000D_ Primary: Anti-PAX2 (bs-1187R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 46 kDa
_x000D_ Observed band size: 47 kDa
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文献和实验该产品被引用文献
[IF=2.586] RUI-NING LIANG. et al. Kidney mesenchymal stem cells alleviate cisplatin-induced kidney injury and apoptosis in rats. TISSUE CELL. 2022 Dec;:101998 IF Rat
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PAX2 Rabbit pAb(bs-1187R)-50ul/100ul/200ul
￥1180 - 2800
