STK11 | LKB1 Rabbit pAb(bs-3948R)-50ul/100ul/200ul产品图
文献支持

STK11 | LKB1 Rabbit pAb(bs-394

8R)-50ul/100ul/200ul
收藏
  • ￥1180 - 2800
  • Bioss已认证
  • bs-3948R
  • 2026年10月13日
    avatar
    北京博奥森生物技术有限公司
    品牌商
    10 年钻石会员

  • 企业认证

    点击 QQ 联系

    • 相关产品推荐更多 >

    万千商家帮你免费找货

    0 人在求购买到急需产品

    相关阅读

    源叶生物 十万种现货试剂速发，品质稳、品类全，实验不再等！Takara 秋促来袭，提取+反转录+定量套装仅 1200 元abinScience 体内级抗体 全年锁价 低至 ￥1200阿拉丁 22万+ 科研级产品现货速发，品质严控、科研无忧
    • 详细信息
    • 文献和实验
    • 技术资料
    • 规格：

      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-3948R
    英文名称LKB1 Rabbit pAb
    中文名称苏氨酸蛋白激酶LKB1抗体
    英文别名LKB1; PJS; Renal carcinoma antigen NY-REN-19; Serine/Threonine Kinase 11; Serine/threonine protein kinase 11; Serine/threonine protein kinase LKB1; Serine/threonine-protein kinase 11; Serine/threonine-protein kinase LKB1; Stk11; STK11_HUMAN; hLKB1; Liver kinase B1; LKB 1; NY REN 19 antigen; Peutz Jeghers syndrome; PJS; Polarization related protein LKB1; Serine/threonine protein kinase STK11; Serine/threonine-protein kinase XEEK1; STK 11.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat, 肝, 肝, 肝, 肝, colon, colon, colon, colon, Cerebrum, Cerebrum, Cerebrum, Cerebrum, colon, colon, colon, breast, breast, breast, breast, human breast carcinoma, human breast carcinoma, human breast carcinoma, human breast carcinoma, colon, colon, colon, uterus, uterus, uterus, uterus, 肝, 肝, 肝, colon, colon, colon, 肝, 肝, 肝, A431 (Chicken, Dog, Cow, Horse, Rabbit, Sheep, Pig)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human LKB1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量48 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Cancer Metabolism > Metabolic signaling pathway > Integration of energy metabolism

    Cancer > Cell cycle > Cell cycle inhibitors > p53 pathway

    Cancer > Oncoproteins/suppressors > Tumor suppressors > p53 pathway

    Cell Biology > Cell Cycle > Cell Cycle Inhibitors > p53

    Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Integration of energy

    亚基Catalytic component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with p53/TP53, SMAD4, STK11IP and WDR6.
    亚细胞定位Nucleus. Cytoplasm. Membrane. Mitochondrion. Note=A small fraction localizes at membranes. Relocates to the cytoplasm when bound to STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta). Translocates to mitochondrion during apoptosis.
    组织特异性Ubiquitously expressed. Strongest expression in testis and fetal liver.
    翻译后修饰Phosphorylated by ATM at Thr-363 following ionizing radiations (IR). Phosphorylation at Ser-428 by RPS6KA1 and/or some PKA is required to inhibit cell growth. Phosphorylation at Ser-428 is also required during neuronal polarization to mediate phosphorylation of BRSK1 and BRSK2.
    相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
    Contains 1 protein kinase domain.
    功能Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, leading to promote their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2: it thereby regulates inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neurons polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate to transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1 Also acts as a mediator p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008].

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{2ug/Test}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LKB1) Polyclonal Antibody, Unconjugated (bs-3948R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LKB1) Polyclonal Antibody, Unconjugated (bs-3948R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LKB1) Polyclonal Antibody, Unconjugated (bs-3948R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片4
    Sample:
    _x000D_ Lane 1: Spleen (Mouse) Lysate at 40 ug
    _x000D_ Lane 2: Lung (Mouse) Lysate at 40 ug
    _x000D_ Primary: Anti-LKB1 (bs-3948R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 50 kD
    _x000D_ Observed band size: 48 kD
    产品细节图片5
    Sample: Hela Cell (Human) Lysate at 40 ug
    _x000D_ Primary: Anti-LKB1 (bs-3948R) at 1/300 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 48 kD
    _x000D_ Observed band size: 50 kD
    _x000D_
    产品细节图片6
    Sample: Testis (Mouse) Lysate at 40 ug
    _x000D_ Primary: Anti-LKB1 (bs-3948R) at 1/300 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 48 kD
    _x000D_ Observed band size: 48 kD
    _x000D_
    产品细节图片7
    P‌‌araformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LKB1) Polyclonal Antibody, Unconjugated (bs-3948R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片8
    P‌‌araformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LKB1) Polyclonal Antibody, Unconjugated (bs-3948R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片9
    P‌‌araformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LKB1) Polyclonal Antibody, Unconjugated (bs-3948R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片10
    P‌‌araformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LKB1) Polyclonal Antibody, Unconjugated (bs-3948R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片11
    P‌‌araformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LKB1) Polyclonal Antibody, Unconjugated (bs-3948R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片12
    P‌‌araformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LKB1) Polyclonal Antibody, Unconjugated (bs-3948R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片13
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LKB1) Polyclonal Antibody, Unconjugated (bs-3948R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片14
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LKB1) Polyclonal Antibody, Unconjugated (bs-3948R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片15
    P‌‌araformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LKB1) Polyclonal Antibody, Unconjugated (bs-3948R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片16
    Blank control（black line）:Hela.
    _x000D_ Primary Antibody (green line): Rabbit Anti-LKB1 antibody (bs-3948R)
    _x000D_ Dilution:2ug/Test;
    _x000D_ Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
    _x000D_ Dilution: 0.5ug/Test.
    _x000D_ Isotype control（orange line）: Normal Rabbit IgG
    _x000D_ Protocol
    _x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

    风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    该产品被引用文献
    [1]LKB1 suppresses KSHV reactivation and promotes primary effusion lymphoma progression.
    PMID:39194241J Virol2024-09-17
    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    LKB1/STK11 Rabbit pAb(WB)
    ￥1990
    上海圻明生物科技有限公司
    2025年07月14日询价
    Rabbit Anti-LKB1/STK11 Polyclonal Antibody
    询价
    卡梅德生物科技（天津）有限公司
    2025年05月29日询价
    Anti-STK11/LKB1
    ￥1
    上海康朗生物科技有限公司
    2026年05月28日询价
    Anti-STK11/LKB1
    ￥1
    上海钰博生物科技有限公司
    2025年07月04日询价
    STK11 Antibody (LKB1)
    ￥4980.18
    安诺伦（北京）生物科技有限公司
    2025年12月03日询价
    文献支持
    STK11 | LKB1 Rabbit pAb(bs-3948R)-50ul/100ul/200ul
    ￥1180 - 2800