Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-GLUR2 Polyclonal Antibody, Unconjugated(bs-1798R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-1798R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1798R |
| 英文名称 | GLUR2 Rabbit pAb |
| 中文名称 | 谷氨酸受体2抗体 |
| 英文别名 | Ionotropic Glutamate receptor 2; Gria2; Glur-2; GluR-B; Glur2; GluR-2; GluR-K2; Glutamate receptor 2; Glutamate receptor ionotropic, AMPA 2; glutamate receptor, ionotropic, N-methyl D-aspartate 1; NMDA receptor subunit; AMPA-selective glutamate receptor 2; AMPA 2; AMPA 2; AMPA selective glutamate receptor 2; AMPA-selective glutamate receptor 2; AMPA2; GluA2; GLUR 2; GluR B; GluR K2; GluR-2; GluR-B; GluR-K2; GLUR2; GLURB; Glutamate receptor 2; Glutamate receptor ionotropic AMPA 2; Glutamate receptor ionotropic; Gria2; GRIA2_HUMAN; HBGR2. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog, Pig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GluR2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 97 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Neurotransmission > Receptors / Channels > GPCR > Glutamate Receptors Neuroscience > Neurotransmitter > Amino Acids > Glutamate |
| 亚基 | Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. May interact with MPP4. Forms a ternary complex with GRIP1 and CSPG4. Interacts with ATAD1 in an ATP-dependent manner. ATAD1-catalyzed ATP hydrolysis disrupts binding to ATAD1 and to GRIP1 and leads to AMPAR complex disassembly. Interacts with PICK1 (via PDZ domain). Interacts with PRKCABP and GRIP2. Interacts with GRIA1 and SYNDIG1. Interacts with LRFN1. Found in a complex with GRIA1, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with CACNG5. |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. |
| 翻译后修饰 | Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity). |
| 相似性 | Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily. |
| 功能 | Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate(AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing(CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg /test} |
Blank control: RSC96(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-AF647(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1ugl in 100 uL1X PBS containing 0.5% BSA(green).
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-AF647(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1ugl in 100 uL1X PBS containing 0.5% BSA(green).
Blank control: RSC96(blue)
Isotype Control Antibody: Rabbit IgG-AF647(orange) ; Primary Antibody Dilution: 5ul in 100uL1X PBS containing 0.5% BSA(green).
Isotype Control Antibody: Rabbit IgG-AF647(orange) ; Primary Antibody Dilution: 5ul in 100uL1X PBS containing 0.5% BSA(green).
Sample: HepG2 Cell Lysate at 30 ug
Primary: Anti-GluR2 (bs-1798R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 97 kD
Observed band size: 117 kD
Primary: Anti-GluR2 (bs-1798R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 97 kD
Observed band size: 117 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLUR2) Polyclonal Antibody, Unconjugated (bs-1798R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLUR2) Polyclonal Antibody, Unconjugated (bs-1798R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control: RSC96(blue)
Isotype Control Antibody: Rabbit IgG-AF647(orange) ; Primary Antibody Dilution: 5ul in 100uL1X PBS containing 0.5% BSA(green).
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[IF={{ 8.58 }}] {Ku, Tingting, et al. "NF-κB-regulated microRNA-574-5p underlies synaptic and cognitive impairment in response to atmospheric PM 2.5 aspiration." Particle and Fibre Toxicology 14.1 (2017): 34.} {WB} {="Mouse"}
[IF={{ 6.105 }}] {Ku et al. NF-κB-regulated microRNA-574-5p underlies synaptic and cognitive impairment in response to atmospheric PM2.5 aspiration. (2017) Part.Fibre.Toxicol. 14:34} {WB} {Mouse}
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GLUR2 Rabbit pAb(bs-1798R)-50ul/100ul/200ul
￥1180 - 2800
