Sample:
HepG2 Cell Lysate at 30 ug
U251 Cell Lysate at 30 ug
Primary: Anti-Dopamine D3 receptor (bs-1743R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 48 kD
- ￥1180 - 2800
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- bs-1743R
- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1743R |
| 英文名称 | Dopamine D3 receptor Rabbit pAb |
| 中文名称 | 多巴胺受体D3抗体 |
| 英文别名 | DRD3; 3dopamine receptor; D; Dopamine receptor D3; DRD3; Dopamine receptor 3; ETM1; FET1; D(3) dopamine receptor; DRD3; DRD3_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:200-800 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse DRD3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 44 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Neurotransmission > Receptors / Channels > GPCR > Dopamine Receptors |
| 亚基 | Interacts with CLIC6. Interacts with GRK4. Interacts with PALM. |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. Note=Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells. |
| 组织特异性 | Brain. |
| 相似性 | Belongs to the G-protein coupled receptor 1 family. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. This receptor is expressed in phylogenetically older regions of the brain, suggesting that it plays a role in cognitive and emotional functions. It is a target for drugs which treat schizophrenia, drug addiction, and Parkinson disease. Alternative splicing of this gene results in multiple transcript variants that would encode different isoforms, although some variants may be subject to nonsense-mediated decay (NMD). Also known as: dopamine D3 receptor; 3 dopamine receptor; D; Dopamine receptor D3; DRD; ETM1; FET1. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:200-800} |
Sample:
Brain (Mouse) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
Primary: Anti-Dopamine D3 receptor (bs-1743R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 48 kD
Brain (Mouse) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
Primary: Anti-Dopamine D3 receptor (bs-1743R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dopamine D3 receptor) Polyclonal Antibody, Unconjugated (bs-1743R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dopamine D3 receptor) Polyclonal Antibody, Unconjugated (bs-1743R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF594) for 90 minutes, and DAPI for nuclei staining.
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文献和实验该产品被引用文献
[IF={{ 6.05 }}] {Andrew Kisling. et al. Loss of Function in Dopamine D3 Receptor Attenuates Left Ventricular Cardiac Fibroblast Migration and Proliferation in vitro. Front Cardiovasc Med. 2021; 8: 732282} {IF} {Mouse}
[IF={{ 5.108 }}] {Yang K et al. Synaptic dopamine release is positively regulated by SNAP-25 that involves in benzo [a] pyrene-induced neurotoxicity. Chemosphere. 2019 Jul 15;237:124378.} {IHC,ICC,WB} {Rat}
[IF={{ 4.831 }}] {Shi L et al. Clioquinol improves motor and non-motor deficits in MPTP-induced monkey model of Parkinson's disease through AKT/mTOR pathway. Aging (Albany NY) . 2020 May 18;12(10):9515-9533.} {WB} {Monkey}
[IF={{ 4.432 }}] {Ting Li. et al. Rotigotine-loaded microspheres exerts the antinociceptive effect via central dopaminergic system. Eur J Pharmacol. 2021 Nov;910:174443} {WB} {Rat}
[IF={{ 4.181 }}] {Bai L et al. m6A demethylase FTO regulates dopaminergic neurotransmission deficits caused by arsenite.Toxicol Sci. 2018 Oct 1;165(2):431-446.} {WB} {Mouse}
相关实验
Genomic Strategies for the Identification of Dopamine Receptor Genes in Zebrafish
In this chapter, we describe the identification and cloning of D2-like dopamine receptor (DR) genes in zebrafish, a vertebrate model genetic organism. To identify DR genes, we performed searches of the zebrafish genomic sequence database
Characterization of Dopamine Receptors
MacKenzie, R.G., VanLeeuwen, D., Pugsley, T.A., Shih, Y.H., Demattos, S., Tang, L., Todd, R.D., and O'Malley, K.L. 1994. Characterization of the human dopamine D3 receptor expressed in transfected cell lines. Eur. J. Pharmacol. 266:79‐85
have been well studied. Elevation of striatal dopamine levels as a consequence of psychostimulant drug administration leads to activation of distinct dopamine receptor subtypes (D1 –D3 ) that are differentially expressed within distinct striatal neuronal populations
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Dopamine D3 receptor Rabbit pAb(bs-1743R)-50ul/100ul/200ul
￥1180 - 2800
