Sample: Kidney (Mouse) Lysate at 40 ug
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Primary: Anti-SRXN1 (bs-8329R) at 1/300 dilution
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Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
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Predicted band size: 15 kD
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Observed band size: 15 kD
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- ￥1180 - 2800
- Bioss
- bs-8329R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-8329R |
| 英文名称 | SRXN1 Rabbit pAb |
| 中文名称 | 抗氧化蛋白1抗体 |
| 英文别名 | C20orf1392; Chromosome 20 open reading frame 139; dJ850E9.2; Npn3; Npn31; SRX1; SRXN 1; Sulfiredoxin 1; Sulfiredoxin 1 homolog(S. cerevisiae); Sulfiredoxin 1 homolog; YKL086W; SRXN1_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SRXN1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 15 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Other Antibodies > Oxidative Stress Metabolism > Pathways and Processes > Redox metabolism > Oxidative stress |
| 亚细胞定位 | Cytoplasmic |
| 组织特异性 | Widely expressed with highest levels in kidney, lung, spleen and thymus. |
| 相似性 | Belongs to the sulfiredoxin family. |
| 功能 | SRXN1 contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxins PRDX1, PRDX2, PRDX3 and PRDX4. It does not act on PRDX5 or PRDX6. SRXN1 may catalyze the reduction in a multi-step process by acting both as a specific phosphotransferase and a thioltransferase. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Sulfiredoxin, also designated Sulfiredoxin-1 and chromosome 20 open reading frame 139 (C20orf139), is a cytoplasmic antioxidant protein involved in signaling through catalytic reduction of oxidative modifications. It regulates peroxiredoxins (PRXs), a family of proteins that reduce hydroperoxides, by reducing the conserved cysteine from sulfinic to sulfenic acid. This impacts the role of PRX in the reduction of other downstream transcription factors and kinase signaling pathways. The Sulfiredoxin protein specifically acts on the PRDX1, PRDX2, PRDX3 and PRDX4 peroxiredoxins, but not on PRDX5 or PRDX6. Sulfiredoxin acts as a phosphotransferase and an athioltransferase and is widely expressed, with highest levels detected in lung, spleen, kidney and thymus tissues. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
_x000D_ Lane 1: Cerebrum (Mouse) Lysate at 40 ug
_x000D_ Lane 2: Cerebrum (Rat) Lysate at 40 ug
_x000D_ Lane 3: HepG2 (Human) Cell Lysate at 30 ug
_x000D_ Lane 4: A549 (Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-SRXN1 (bs-8329R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 15 kD
_x000D_ Observed band size: 15 kD
_x000D_ Lane 1: Cerebrum (Mouse) Lysate at 40 ug
_x000D_ Lane 2: Cerebrum (Rat) Lysate at 40 ug
_x000D_ Lane 3: HepG2 (Human) Cell Lysate at 30 ug
_x000D_ Lane 4: A549 (Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-SRXN1 (bs-8329R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 15 kD
_x000D_ Observed band size: 15 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (SRXN1) Polyclonal/MonoclonalAntibody, Unconjugated (bs-8329R) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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SRXN1 | SRX1 Rabbit pAb(bs-8329R)-50ul/100ul/200ul
￥1180 - 2800
