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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6419R |
| 英文名称 | phospho-PP2A alpha+beta (Tyr307) Rabbit pAb |
| 中文名称 | 磷酸化蛋白磷酸酶2A（PP2Aα）抗体 |
| 英文别名 | p-PP2A-Cα/β; PP2A alpha(phospho Y307); p-PP2A alpha(Y307); PP2A A; PP2A alpha; PP2A B; PP2A beta; PP2Ac; PP2C alpha; PP2C beta; PP2CA; PP2CB; PPP2CA; PPP2CB; Protein phosphatase 2(formerly 2A) catalytic subunit alpha isoform; Protein phosphatase 2(formerly 2A) catalytic subunit beta isoform; Protein phosphatase 2; Protein phosphatase 2 catalytic subunit alpha isoform; Protein phosphatase 2 catalytic subunit beta isoform; Protein Phosphatase 2A; Protein phosphatase type 2A catalytic subunit; Replication protein C; RP C; Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform; Serine/threonine protein phosphatase 2A catalytic subunit beta isoform; Serine/threonine protein phosphatase. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Chicken, Dog, Pig, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from human PPP2CA around the phosphorylation site of Tyr307 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 34 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell cycle > Kinases/phosphatases > Phosphatases Cardiovascular > Heart > Contractility > Contractile Proteins > Troponin Cell Biology > Cell Cycle > Kinases/Phosphatases Cell Biology > Cell Cycle > Kinases/Phosphatases > Phosphatases Epigenetics and Nuclear Signaling > Cell cycle > Kinases/Phosphatases Neuroscience > Neurotransmission > Intracellular Signaling > Phosphatases Signal Transduction > Protein Phosphorylation > Ser / Thr Phosphatases Stem Cells > Signaling Pathways > TGF beta > Cytoplasmic Stem Cells > Signaling Pathways > Wnt > Cytoplasmic |
| 亚基 | PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with NXN; the interaction is direct (By similarity). Interacts with TP53, SGOL1 and SGOL2. Interacts with AXIN1; the interaction dephosphorylates AXIN1. Interacts with PIM3; this interaction promotes dephosphorylation, ubiquitination and proteasomal degradation of PIM3. Interacts with RAF1. |
| 亚细胞定位 | Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. Note=In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity). |
| 翻译后修饰 | Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle._x000D_
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. |
| 相似性 | Belongs to the PPP phosphatase family. PP-1 subfamily. |
| 功能 | PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
_x000D_ Hela(Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-phospho-PP2A alpha+beta(Tyr307) (bs-6419R) at 1/500 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 34 kD
_x000D_ Observed band size: 34 kD
_x000D_
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文献和实验[IF=7.285] Shen, Liang. et al. Angiotensin Type 2 Receptor Pharmacological Agonist Relieves Neurocognitive Deficits via Reducing Neuroinflammation and Microglial Engulfment of Dendritic Spines. J NEUROIMMUNE PHARM. 2022 Dec;:1-17 WB Mouse
ITGA4（CD49D）信号通路、病理作用与靶向治疗药物研究梳理
整合素alpha 4（ITGA4），又称CD49D，是整合素家族的关键亚基。它通过与β1或β7亚基结合，形成功能性异二聚体α4β1（VLA-4）和α4β7，在免疫细胞的黏附、运输及迁移中扮演核心角色。VLA-4还能介导白细胞在活化内皮细胞上的聚集。鉴于ITGA4在多发性硬化、炎症性肠病等多种自身免疫性疾病的病理过程中发挥关键作用，它已成为治疗干预的重要靶点。 ITGA4的表达分布 ITGA4主要分布于巨核细胞、红细胞、干细胞、NK细胞以及T细胞中。在T细胞亚群中，CD49D主要在CD
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
andquantified ratios of phosphorylation relative to total proteinare provided. Figure 4.1b shows analysis of the same celllysates using phospho-β-catenin and p-CRMP2, two establishedtargets of GSK3. Note that only the phosphorylationof β-catenin is reduced
rabbit serum albumin 兔血清白蛋白 R-Sc Reed-Sternberg's cell 瑞-司氏细胞 Z/R Reinheit Zahl 光密度比值 SAV small agranular vesicle 无颗粒小泡 SBA soybean agglutinin 大豆凝集素 SC secretory component 分泌成份 SCV small clear vesicle 清亮小泡 SDS sodium
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