KLK1 Rabbit pAb(bs-1963R)-50ul/100ul/200ul
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KLK1 Rabbit pAb(bs-1963R)-50ul

/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-1963R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-1963R
    英文名称KLK1 Rabbit pAb
    中文名称激肽释放酶1抗体
    英文别名Kallikrein 1; Kallikrein-1; hK1; Klk6; KLKR; Glandular kallikrein 1; hK 1; Kallikrein serine protease 1; Kidney/pancreas/salivary gland kallikrein; Klk 6; Tissue kallikrein; Klk1b3; Klk1c1; Ngfg; rGK-1; preprokallikrein; KLK1_HUMAN.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Dog, Pig, Horse, Rabbit)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human KLK1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量26 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Invasion/microenvironment > Angiogenesis > ECM enzymes > Kallikreins

    Cancer > Invasion/microenvironment > ECM > Extracellular matrix > Kallikreins

    Cardiovascular > Blood > Coagulation > Intrinsic

    Cardiovascular > Blood > Serum Proteins

    Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes

    Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes > Serine protease > Kallikreins

    Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > ECM Enzymes > Kallikreins

    组织特异性Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
    翻译后修饰The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.
    相似性Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.
    功能Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. [provided by RefSeq, Jul 2008]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    KLK1 Rabbit pAb(bs-1963R)-50ul
    P‌‌araformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (bs-1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    KLK1 Rabbit pAb(bs-1963R)-50ul
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (bs-1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    KLK1 Rabbit pAb(bs-1963R)-50ul
    Sample:
    Lane 1: Human K562 cell lysates
    Lane 2: Human SW480 cell lysates
    Primary: Anti-KLK1 (bs-1963R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 26 kD
    Observed band size: 30 kD
    KLK1 Rabbit pAb(bs-1963R)-50ul
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (bs-1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    KLK1 Rabbit pAb(bs-1963R)-50ul
    P‌‌araformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (bs-1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    KLK1 Rabbit pAb(bs-1963R)-50ul
    P‌‌araformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (bs-1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    KLK1 Rabbit pAb(bs-1963R)-50ul
    P‌‌araformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK1) Polyclonal Antibody, Unconjugated (bs-1963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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    该产品被引用文献

    [IF={{ 0 }}] {Morrison MD et al. Identifying Candidate Biomarkers for Pleomorphic Adenoma: A Case-Control Study. Head Neck Pathol. 2019 Sep;13(3):286-297. } {IHC} {Human}

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    文献支持
    KLK1 Rabbit pAb(bs-1963R)-50ul/100ul/200ul
    ￥1180 - 2800