Sample:
Lane 1: Recombinant protein A
Primary: Anti- Protein A (bs-0362R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40-60 kDa
Observed band size: 41 kDa
- ￥1180 - 2800
- Bioss
- bs-0362R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0362R |
| 英文名称 | Protein A Rabbit pAb |
| 中文名称 | 重组金黄色葡萄球菌蛋白A抗体 |
| 英文别名 | Immunoglobulin G binding protein A; Staphylococcal protein A; SPA; gG-binding protein A; SPA_STAA8. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | S.aureus |
| 抗体来源 | Rabbit |
| 免疫原 | Recombinant protein A |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 40-60 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Tags & Cell Markers > Fusion / Marker Proteins > Protein A |
| 亚细胞定位 | Secreted; cell wall. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验该产品被引用文献
[IF={{ 9.518 }}] {Wu J et al. A laser-induced breakdown spectroscopy-integrated lateral flow strip (LIBS-LFS) sensor for rapid detection of pathogen. Biosens Bioelectron. 2019 Jul 13;142:111508.} {Other} {S. aureus}
[IF={{ 3.412 }}] {Lirui Ge. et al. Lateral Flow Immunoassay for Visible Detection of Human Brucellosis Based on Blue Silica Nanoparticles. Front Vet Sci. 2021; 8: 771341} {Other} {Other}
[IF={{ 1.134 }}] {Wen Wu. et al. Ti3C2-MXene-assisted signal amplification for sensitive and selective surface plasmon resonance biosensing of biomarker. Chinese J Anal Chem. 2021 Nov;:} {Other} {}
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Protein A Rabbit pAb(bs-0362R)-50ul/100ul/200ul
￥1180 - 2800
