Tissue/cell: rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-BMP2 Polyclonal Antibody, Unconjugated(bs-10696R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-10696R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-10696R |
| 英文名称 | BMP2 Rabbit pAb |
| 中文名称 | 骨形态发生蛋白2抗体 |
| 英文别名 | Bone morphogenetic protein 2; BMP 2; BMP 2A; BMP2A; BMP-2; BMP2_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken, Dog, Pig, Cow, Horse, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human BMP2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 13/44 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Growth factors > TGF Cardiovascular > Angiogenesis > Growth Factors > TGF Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > Structures > Bone Signal Transduction > Growth Factors/Hormones > TGF Stem Cells > Neural Stem Cells > Extracellular Stem Cells > Signaling Pathways > TGF beta > Secreted |
| 亚基 | Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with GREM2, RGMA, RGMB and RGMC. Interacts with ASPN. |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine. |
| 相似性 | Belongs to the TGF-beta family. |
| 功能 | Induces cartilage and bone formation. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily of secreted growth factors. It is a disulfide-linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
A431(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-BMP2 (bs-10696R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/44 kD
Observed band size: 64 kD
A431(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-BMP2 (bs-10696R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/44 kD
Observed band size: 64 kD
Sample:
Stomach (Mouse) Lysate at 40 ug
Primary: Anti-BMP2 (bs-10696R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/44 kD
Observed band size: 59 kD
Stomach (Mouse) Lysate at 40 ug
Primary: Anti-BMP2 (bs-10696R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/44 kD
Observed band size: 59 kD
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文献和实验该产品被引用文献
[IF={{ 10.7 }}] {Yingqi Chen. et al.Biofunctional supramolecular injectable hydrogel with spongy-like metal-organic coordination for effective repair of critical-sized calvarial defects.asian j pharm sci.2025 Feb;20(1):100988.} {IHC} {Rat}
[IF={{ 10.2 }}] {Li Fu-Xing-Zi. et al. Cold exposure protects against medial arterial calcification development via autophagy. J NANOBIOTECHNOL. 2023 Dec;21(1):1-22} {WB} {Mouse}
[IF={{ 10.2 }}] {Zheng Ming-Hui. et al. Vascular wall microenvironment: exosomes secreted by adventitial fibroblasts induced vascular calcification. J NANOBIOTECHNOL. 2023 Dec;21(1):1-21} {WB,IHC} {Mouse}
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BMP2 Rabbit pAb(bs-10696R)-50ul/100ul/200ul
￥1180 - 2800
